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| GROWTH SUPPRESSION OF HUMAN HEAD AND NECK-CANCER CELLS BY THE INTRODUCTION OF A WILD-TYPE P53 GENE VIA A RECOMBINANT ADENOVIRUS |
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| Author(s): LIU TJ, ZHANG WW, TAYLOR DL, ROTH JA, GOEPFERT H, CLAYMAN GL |
| Source: CANCER RESEARCH Volume: 54 Issue: 14 Pages: 3662-3667 Published: JUL 15 1994 |
| Times Cited: 254 References: 24 |
| Abstract: Mutations of the p53 gene constitute one of the most frequent genetic alterations in squamous cell carcinoma of the head and neck (SCCHN). In this study, we introduced wild-type p53 into two separate SCCHN cell lines via a recombinant adenoviral vector, Ad5CMV-p53. Northern blotting showed that following infection by the wild-type p53 adenovirus (Ad5CMV-p53), cells produced up to l0-fold higher levels of exogenous p53 mRNA than cells treated with vector only (without p53). Western blotting showed that the increased levels of p53 protein produced in the Ad5CMV-pS3-infected cells were a reflection of p53 mRNA expression. In vitro growth assays revealed growth arrest following Ad5CMV-p53 infection as well as cell morphological changes consistent with apoptosis. In vivo studies in nude mice with established s.c. squamous carcinoma nodules showed that tumor volumes were significantly reduced in mice that received peritumoral infiltration of Ad5CMV-p53. These data suggest that Ad5CMV-p53 may be further developed as a potential novel therapeutic agent for SCCHN since introduction of wild-type p53 into SCCHN cell lines attenuates their replication and tumor growth. |
| Document Type: Note |
| Language: English |
Addresses:
1. UNIV TEXAS, MD ANDERSON CANC CTR, DEPT HEAD & NECK SURG, THORAC MOLEC ONCOL SECT, HOUSTON, TX 77030 USA
2. UNIV TEXAS, MD ANDERSON CANC CTR, DEPT CARDIOVASC & THORAC SURG, HOUSTON, TX 77030 USA |
| Publisher: AMER ASSOC CANCER RESEARCH, PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 |
| Subject Category: Oncology |
| IDS Number: NV956 |
| ISSN: 0008-5472 |
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