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| A NOVEL HETERODIMERIC TRANSFERRIN RECEPTOR ENCODED BY A PAIR OF VSG EXPRESSION SITE-ASSOCIATED GENES IN TRYPANOSOMA-BRUCEI |
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| Author(s): SALMON D, GEUSKENS M, HANOCQ F, HANOCQQUERTIER J, NOLAN D, RUBEN L, PAYS E |
| Source: CELL Volume: 78 Issue: 1 Pages: 75-86 Published: JUL 15 1994 |
| Times Cited: 103 References: 38 |
| Abstract: In T. brucei, a transferrin-binding protein has been found to share sequence homology with pESAG-7 and -6, the products of two related genes present in the VSG gene polycistronic transcription unit. When expressed in Xenopus oocytes, they appear as N-glycosylated proteins secreted in the medium (pESAG-7) and GPI anchored to the membrane (pESAG-6). These proteins are able to homo- or heterodimerize, probably through association in the same orientation. Only heterodimers can bind Tf, possibly two molecules per dimer. A comparison of Tf binding to pESAG-7/6-expressing oocytes and trypanosomes suggests that pESAG-7/6 is the Tf receptor of the parasite. In trypanosomes, the majority of pESAG-7/6 is released from the membrane and associates, together with Tf, with a glycosylated matrix present in the lumen of the flagellar pocket. Both pESAG-7/6 and Tf are internalized via coated pits and vesicles. These observations suggest a novel mode of Tf binding and uptake in trypanosomes. |
| Document Type: Article |
| Language: English |
| Reprint Address: SALMON, D (reprint author), UNIV BRUSSELS, DEPT MOLEC BIOL, B-1640 RHODE ST GENESE, BELGIUM |
Addresses:
1. SO METHODIST UNIV, DEPT BIOL SCI, DALLAS, TX 75275 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: NX328 |
| ISSN: 0092-8674 |
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