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| NUCLEAR-PROTEIN CBP IS A COACTIVATOR FOR THE TRANSCRIPTION FACTOR CREB |
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| Author(s): KWOK RPS, LUNDBLAD JR, CHRIVIA JC, RICHARDS JP, BACHINGER HP, BRENNAN RG, ROBERTS SGE, GREEN MR, GOODMAN RH |
| Source: NATURE Volume: 370 Issue: 6486 Pages: 223-226 Published: JUL 21 1994 |
| Times Cited: 1,062 References: 19 |
| Abstract: THE transcription factor CREB binds to a DNA element known as the cAMP-regulated enhancer (CRE)(1-5). CREB is activated through phosphorylation by protein kinase A (PKA)(6), but precisely how phosphorylation stimulates CREB function is unknown. One model is that phosphorylation may allow the recruitment of coactivators which then interact with basal transcription factors. We have previously identified a nuclear protein of M(r) 265K, CBP, that binds specifically to the PKA-phosphorylated form of CREB(7). We have used fluorescence anisotropy measurements to define the equilibrium binding parameters of the phosphoCREB:CBP interaction and report here that CBP can activate transcription through a region in its carboxy terminus. The activation,domain of CBP interacts with the basal transcription factor TFIIB through a domain that is conserved in the yeast coactivator ADA-1 (ref. 8). Consistent with its role as a coactivator, CBP augments the activity of phosphorylated CREB to activate transcription of cAM-responsive genes. |
| Document Type: Article |
| Language: English |
Addresses:
1. OREGON HLTH SCI UNIV, VOLLUM INST, PORTLAND, OR 97201 USA
2. OREGON HLTH SCI UNIV, DEPT BIOCHEM, PORTLAND, OR 97201 USA
3. ST LOUIS UNIV, DEPT PHARMACOL & PHYSIOL SCI, ST LOUIS, MO 63014 USA
4. SHRINERS HOSP CRIPPLED CHILDRENS, PORTLAND, OR 97201 USA
5. UNIV MASSACHUSETTS, MED CTR, PROGRAM MOLEC MED, WORCESTER, MA 01605 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: NX971 |
| ISSN: 0028-0836 |
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