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| VISUALIZATION OF A-BETA-42(43) AND A-BETA-40 IN SENILE PLAQUES WITH END-SPECIFIC A-BETA MONOCLONALS - EVIDENCE THAT AN INITIALLY DEPOSITED SPECIES IS A-BETA-42(43) |
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| Author(s): IWATSUBO T, ODAKA A, SUZUKI N, MIZUSAWA H, NUKINA N, IHARA Y |
| Source: NEURON Volume: 13 Issue: 1 Pages: 45-53 Published: JUL 1994 |
| Times Cited: 842 References: 41 |
| Abstract: To learn about the carboxy-terminal extent of amyloid beta-protein (A beta) composition of senile plaques (SPs) in the brain affected with Alzheimer's disease (AD), we employed two end-specific monoclonal antibodies as immunocytochemical probes: one is specific for A beta 40, the carboxyl terminus of A beta 1-40, while the other is specific for A beta 42(43). In the AD cortex, all SPs that were labeled with an authentic antibody were A beta 42(43) positive, while only one-third of which, on the average, were A beta 40 positive. There was a strong correlation between A beta 40 positivity and mature plaques. Two familial AD cortices with the mutation of beta-amyloid protein precursor 717 (beta APP717) (Val to Ile) showed a remarkable predominance of A beta 42(43)-positive, A beta 40-negative plaques. Diffuse plaques, representing the earliest stage of A beta deposition, were exclusively positive for A beta 42(43), but completely negative for A beta 40. |
| Document Type: Article |
| Language: English |
| Reprint Address: IWATSUBO, T (reprint author), UNIV TOKYO, FAC PHARMACEUT SCI, DEPT NEUROPATHOL & NEUROSCI, BUNKYO KU, TOKYO 113, JAPAN |
Addresses:
1. UNIV TOKYO, FAC MED, INST BRAIN RES, DEPT NEUROL, BUNKYO KU, TOKYO 113, JAPAN
2. UNIV TOKYO, FAC MED, INST BRAIN RES, DEPT NEUROPATHOL, BUNKYO KU, TOKYO 113, JAPAN
3. TAKEDA CHEM IND, DIV DISCOVERY RES, TSUKUBA, IBARAKI 30042 JAPAN
4. UNIV TSUKUBA, DEPT NEUROL, TSUKUBA, IBARAKI 305 JAPAN |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Neurosciences |
| IDS Number: NZ247 |
| ISSN: 0896-6273 |
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