| NMR SOLUTION STRUCTURE OF A PEPTIDE NUCLEIC-ACID COMPLEXED WITH RNA |
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| Author(s): BROWN SC, THOMSON SA, VEAL JM, DAVIS DG |
| Source: SCIENCE Volume: 265 Issue: 5173 Pages: 777-780 Published: AUG 5 1994 |
| Times Cited: 159 References: 34 |
| Abstract: Peptide nucleic acids (PNA) incorporating nucleic acid bases into an achiral polyamide backbone bind to DNA in a sequence-dependent manner. The structure of a PNA-ribonucleic acid (RNA) complex was determined with nuclear magnetic resonance methods. A hexameric PNA formed a 1:1 complex with a complementary RNA that is an antiparallel, right-handed double helix with Watson-Crick base pairing similar to the ''A'' form structure of RNA duplexes. The achiral PNA backbone assumed a distinct conformation upon binding that differed from previously proposed models and provides a basis for further structure-based design of antisense agents. |
| Document Type: Article |
| Language: English |
| Reprint Address: BROWN, SC (reprint author), GLAXO INC, RES INST, RES TRIANGLE PK, NC 27709 USA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: PA372 |
| ISSN: 0036-8075 |