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| NUCLEOSOME DISRUPTION AND ENHANCEMENT OF ACTIVATOR BINDING BY A HUMAN SW1/SNF COMPLEX |
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| Author(s): KWON H, IMBALZANO AN, KHAVARI PA, KINGSTON RE, GREEN MR |
| Source: NATURE Volume: 370 Issue: 6489 Pages: 477-481 Published: AUG 11 1994 |
| Times Cited: 469 References: 24 |
| Abstract: CHROMATIN structure can affect the transcriptional activity of eukaryotic structural genes by blocking access of sequence-specific activator proteins (activators) to their promoter-binding sites(1). For example, the DNA-binding domain of the yeast GAL4 protein interacts very poorly with nucleosome cores compared with naked DNA(2) (and see below), and binding of other activators is even more strongly inhibited(2,3). The way in which activators bind to nucleosomal DNA is therefore a critical aspect of transcriptional activation. Genetic studies have suggested that the multi-component SWI/SNF complex of Saccharomyces cerevisiae facilitates transcription by altering the structure of the chromatin(4,5). Here we identify and partially purify a human homologue of the yeast SWI/SNF complex (hSWI/SNF complex). We show that a partially purified hSWI/SNF complex mediates the ATP-dependent disruption of a nucleosome, thereby enabling the activators, GAL4-VP16 and GAL4-AH, to bind within a nucleosome core. We conclude that the hSWI/SNF complex acts directly to reorganize chromatin structure so as to facilitate binding of transcription factors. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV MASSACHUSETTS, SCH MED, HOWARD HUGHES MED INST, PROGRAM MOLEC MED, WORCESTER, MA 01605 USA
2. MASSACHUSETTS GEN HOSP, DEPT MOLEC BIOL, BOSTON, MA 02114 USA
3. HARVARD UNIV, SCH MED, DEPT GENET, BOSTON, MA 02115 USA
4. STANFORD UNIV, CTR MOILEC & GENET MED, HOWARD HUGHES MED INST, STANFORD, CA 94305 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: PB407 |
| ISSN: 0028-0836 |
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