| GENETIC-TRANSFORMATION AND REGENERATION OF TRANSGENIC PLANTS IN GRAPEVINE (VITIS-RUPESTRIS S) |
|
|
| Author(s): MARTINELLI L, MANDOLINO G |
| Source: THEORETICAL AND APPLIED GENETICS Volume: 88 Issue: 6-7 Pages: 621-628 Published: AUG 1994 |
| Times Cited: 38 References: 36 |
| Abstract: Isolated somatic embryos from petiole-derived callus cultures of Vitis rupestris Scheele have been employed in experiments on genetic transformation. Co-cultivation of somatic embryos during embryogenesis induction with Agrobacterium tumefaciens strain LBA4404, which contains the plasmid pBI121 carrying the neomycin -phosphotranspherase and the beta-glucuronidase genes, produced transformed cellular lines capable of recurrent somatic embryogenesis. Precocious selection for high levels of kanamycin (100 mgl(-1)) was an important part of our transformation protocol. Transformed lines still have strong beta-glucuronidase expression as well as stable insertion of the marker genes after 3 years of in-vitro culture, during which they have maintained their capacity to organize secondary embryos and to regenerate transgenic plants with an agreeable efficiency (13%). |
| Document Type: Article |
| Language: English |
| Reprint Address: MARTINELLI, L (reprint author), IST AGR, VIA MACH 1, I-38010 SAN MICHELE ADIGE, ITALY |
Addresses:
1. IST SPERIMENTALE COLTURE IND, BOLOGNA, ITALY |
| Publisher: SPRINGER VERLAG, 175 FIFTH AVE, NEW YORK, NY 10010 |
| Subject Category: Agronomy; Plant Sciences; Genetics & Heredity; Horticulture |
| IDS Number: PB681 |
| ISSN: 0040-5752 |