| | |  | | | | Record from Web of Science® | | | | | | |
| NETRINS ARE DIFFUSIBLE CHEMOTROPIC FACTORS FOR COMMISSURAL AXONS IN THE EMBRYONIC SPINAL-CORD |
|
|
| Author(s): KENNEDY TE, SERAFINI T, DELATORRE JR, TESSIERLAVIGNE M |
| Source: CELL Volume: 78 Issue: 3 Pages: 425-435 Published: AUG 12 1994 |
| Times Cited: 676 References: 36 |
| Abstract: The guidance of axons to their targets in the developing nervous system is believed to involve diffusible chemotropic factors secreted by target cells. Floor plate cells at the ventral midline of the spinal cord secrete a diffusible factor or factors that promotes the outgrowth of spinal commissural axons and attracts these axons in vitro. Two membrane-associated proteins isolated from brain, netrin-1 and netrin-2, possess commissural axon outgrowth-promoting activity. We show here that netrin-1 RNA is expressed by floor plate cells, whereas netrin-2 RNA is detected at lower levels in the ventral two-thirds of the spinal cord, but not the floor plate. Heterologous cells expressing recombinant netrin-1 or netrin-2 secrete diffusible farms of the proteins and can attract commissural axons at a distance. These results show that netrin-1 is a chemotropic factor expressed by floor plate cells and suggest that the two netrin proteins guide commissural axons in the developing spinal cord. |
| Document Type: Article |
| Language: English |
| Reprint Address: KENNEDY, TE (reprint author), UNIV CALIF SAN FRANCISCO, HOWARD HUGHES MED INST, DEPT ANAT, CELL BIOL PROGRAM, SAN FRANCISCO, CA 94143 USA |
Addresses:
1. UNIV CALIF SAN FRANCISCO, HOWARD HUGHES MED INST, DEPT ANAT, PROGRAM DEV BIOL, SAN FRANCISCO, CA 94143 USA
2. UNIV CALIF SAN FRANCISCO, HOWARD HUGHES MED INST, DEPT ANAT, PROGRAM NEUROSCI, SAN FRANCISCO, CA 94143 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: PC405 |
| ISSN: 0092-8674 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |