| | |  | | | | Record from Web of Science® | | | | | | |
| CHARACTERIZATION OF THE GENOMIC STRUCTURE, CHROMOSOMAL LOCATION AND PROMOTER OF HUMAN PROSTAGLANDIN-H SYNTHASE-2 GENE |
|
|
| Author(s): TAZAWA R, XU XM, WU KK, WANG LH |
| Source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 203 Issue: 1 Pages: 190-199 Published: AUG 30 1994 |
| Times Cited: 187 References: 41 |
| Abstract: Prostaglandin H synthase (PGHS) is the rate-limiting enzyme in the conversion of arachidonic acid to prostanoids. The human PGHS has two isoforms. PGHS-1 is a house keeping gene whereas PGHS-2 is an inducible gene. We reported here the isolation of the entire PGHS-2 gene and its 5'-flanking region from a human bacteriophage P1 genomic library. The gene containing 10 exons is 7.5 kb in length and located at chromosome 1. The transcriptional start site was mapped at 134 bases upstream from the ATG start codon. Nucleotide sequence of 1.8 kb promoter region contains a TATA box and a number of potential regulatory elements including CRE, NF-kappa B, Sp1 and AP2 sites. Studies of the promoter activity showed that the first 460 nucleotides of 5'-flanking region efficiently drove transcription of the luciferase reporter gene in human umbilical vein endothelial cells upon stimulation with phorbor ester. (C) 1994 Academic Press, Inc. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV TEXAS, DEPT INTERNAL MED, DIV HEMATOL, HOUSTON, TX 77225 USA
2. UNIV TEXAS, DEPT INTERNAL MED, VASC BIOL RES CTR, HOUSTON, TX 77030 USA |
| Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 |
| Subject Category: Biochemistry & Molecular Biology; Biophysics |
| IDS Number: PD473 |
| ISSN: 0006-291X |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |