| CHARACTERIZATION OF HUMAN SERUM-RESISTANT AND SERUM-SENSITIVE CLONES FROM A SINGLE TRYPANOSOMA-BRUCEI-GAMBIENSE PARENTAL CLONE |
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| Author(s): ORTIZ JC, SECHELSKI JB, SEED JR |
| Source: JOURNAL OF PARASITOLOGY Volume: 80 Issue: 4 Pages: 550-557 Published: AUG 1994 |
| Times Cited: 6 References: 30 |
| Abstract: A protocol was developed to select clones of Trypanosoma brucei gambiense having different levels of resistance to normal human serum. Human serum-resistant clones were selected from a single parental clone by continuous serum treatment of infected immunosuppressed mice. Human serum-sensitive revertant clones were also obtained by continuous passage of resistant clones in immunosuppressed mice but without human serum pressure. It has been demonstrated that our trypanosome clones express distinct but stable levels of resistance. The variant antigenic type of each clone was characterized serologically and by 1-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. After selective pressure with human serum, variant antigen-type differences always occurred among clones in which different human serum susceptibilities were found. The work reported here demonstrates that in our T. brucei gambiense immunosuppressed mouse model there is a predictable association between variant antigen type and human serum resistance. |
| Document Type: Article |
| Language: English |
| Reprint Address: ORTIZ, JC (reprint author), CTR INT ENTRENAMIENTO & INVEST MED, BIOCHEM UNIT, APARTADO AEREO 5390, CALI, COLOMBIA |
| Publisher: AMER SOC PARASITOLOGISTS, 810 EAST 10TH STREET, LAWRENCE, KS 66044 |
| Subject Category: Parasitology |
| IDS Number: PE284 |
| ISSN: 0022-3395 |