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| MOT1, A GLOBAL REPRESSOR OF RNA-POLYMERASE-II TRANSCRIPTION, INHIBITS TBP BINDING TO DNA BY AN ATP-DEPENDENT MECHANISM |
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| Author(s): AUBLE DT, HANSEN KE, MUELLER CGF, LANE WS, THORNER J, HAHN S |
| Source: GENES & DEVELOPMENT Volume: 8 Issue: 16 Pages: 1920-1934 Published: AUG 15 1994 |
| Times Cited: 224 References: 81 |
| Abstract: Basal transcription of many genes in yeast is repressed by Mot1, an essential protein which is a member of the Snf2/Swi2 family of conserved nuclear factors. ADI is an ATP dependent inhibitor of TATA binding protein (TBP) binding to DNA that inhibits transcription in vitro. Here we demonstrate that ADI is encoded by the MOT1 gene. Mutation of MOT1 abolishes ADI activity and derepresses basal transcription in vitro and in vivo. Recombinant Motl removes TBP from DNA and Motl contains an ATPase activity which is essential for its function. Genetic interactions between Mot1 and TBP indicate that their functions are interlinked in vivo. These results provide a general model for understanding the mechanism of action of a large family of nuclear factors involved in processes such as transcription and DNA repair. . |
| Document Type: Article |
| Language: English |
| Reprint Address: AUBLE, DT (reprint author), FRED HUTCHINSON CANC RES CTR, 1124 COLUMBIA ST, SEATTLE, WA 98104 USA |
Addresses:
1. UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, DIV BIOCHEM & MOLEC BIOL, BERKELEY, CA 94720 USA
2. HARVARD UNIV, HARVARD MICROCHEM FACIL, CAMBRIDGE, MA 02138 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 |
| Subject Category: Cell Biology; Developmental Biology; Genetics & Heredity |
| IDS Number: PE731 |
| ISSN: 0890-9369 |
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