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| MICROSATELLITE INSTABILITY IN ULCERATIVE COLITIS-ASSOCIATED COLORECTAL DYSPLASIAS AND CANCERS |
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| Author(s): SUZUKI H, HARPAZ N, TARMIN L, YIN J, JIANG HY, BELL JD, HONTANOSAS M, GROISMAN GM, ABRAHAM JM, MELTZER SJ |
| Source: CANCER RESEARCH Volume: 54 Issue: 18 Pages: 4841-4844 Published: SEP 15 1994 |
| Times Cited: 118 References: 34 |
| Abstract: Microsatellites are short nucleotide repeat sequences present throughout the human genome. Alterations of microsatellites, comprising extra or missing copies of these sequences, have been termed microsatellite instability. This abnormality occurs in sporadic and hereditary adenocarcinomas of the proximal colon, as well as in many other tumor types. We determined whether microsatellite instability occurred in ulcerative colitis-associated cancers or precancerous dysplasias. Sixty-three patients were evaluated, consisting of 188 samples of genomic DNA (63 normal controls, 68 cancers, 52 dysplasias, and 5 adjacent tissues) at loci D2S119, D2S123, D2S147, D10S197, and D11S904. Multiplex polymerase chain reaction was performed using one radiolabeled nucleotide, and the products were electrophoresed on denaturing polyacrylamide gels. Seventeen of the 63 patients (27%) possessed lesions showing instability at 1 or more loci. Fourteen of 68 tumor samples (21%) and ten of 52 dysplasias (19%) displayed instability. There was no tendency for a greater number of loci to manifest instability in more advanced lesions. Neither anatomic location nor loss of heterozygosity at the p53 locus were associated with microsatellite instability by 2-way table analysis. These data support a role for defective DNA repair in the generation of a subset of both early and advanced ulcerative colitis-associated colorectal neoplastic lesions. |
| Document Type: Note |
| Language: English |
Addresses:
1. UNIV MARYLAND, SCH MED, DEPT MED, DIV GASTROENTEROL, BALTIMORE, MD 21201 USA
2. BALTIMORE VET AFFAIRS HOSP, BALTIMORE, MD 21201 USA
3. CUNY, MT SINAI SCH MED, DEPT PATHOL, NEW YORK, NY USA |
| Publisher: AMER ASSOC CANCER RESEARCH, PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 |
| Subject Category: Oncology |
| IDS Number: PF791 |
| ISSN: 0008-5472 |
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