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| IDENTIFICATION OF THE MN ANTIGEN AS A DIAGNOSTIC BIOMARKER OF CERVICAL INTRAEPITHELIAL SQUAMOUS AND GLANDULAR NEOPLASIA AND CERVICAL CARCINOMAS |
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| Author(s): LIAO SY, BREWER C, ZAVADA J, PASTOREK J, PASTOREKOVA S, MANETTA A, BERMAN ML, DISAIA PJ, STANBRIDGE EJ |
| Source: AMERICAN JOURNAL OF PATHOLOGY Volume: 145 Issue: 3 Pages: 598-609 Published: SEP 1994 |
| Times Cited: 127 References: 25 |
| Abstract: A new tumor-associated antigen, MN, has been identified whose expression correlated with the tumorigenic phenotype of HeLa X fibroblast somatic cell hybrids. Because HeLa is a cell line derived from a cervical carcinoma, we investigated the diagnostic utility of MN expression in cervical neoplasia. It was found that normal cervical tissue does not express the antigen, or does so in occasional focal areas of weakly staining reserve cells. In contrast, significant immunoreactivity (immunoperoxidase staining of paraffin-embedded sections) was observed in cervical intraepithelial neoplasias, adenocarcinoma in situ, and frank carcinomas, both squamous cell and adenocarcinoma. Varying patterns of immunoreactivity were observed and were characterized as diffuse, diffuse/focal, or focal. Greater than 90% of dysplastic or malignant tissues showed immunoreactivity. An interesting observation was that normal cervical tissue associated with cervical intraepithelial neoplasias, or adenocarcinoma in situ showed a staining pattern in the reserve cells and/or normal columnar cells that approximated the level of intensity exhibited by the dysplastic tissue. These results indicate that MN antigen expression has potential utility as a biomarker of cervical neoplasms. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV CALIF IRVINE, COLL MED, DEPT MICROBIOL & MOLEC GENET, IRVINE, CA 92717 USA
2. ST JOSEPH HOSP, DEPT PATHOL, ORANGE, CA USA
3. CHILDRENS HOSP ORANGE CTY, ORANGE, CA 92668 USA
4. UNIV CALIF IRVINE, COLL MED, DEPT OBSTET & GYNECOL, IRVINE, CA USA
5. ACAD SCI CZECH REPUBL, INST MOLEC GENET, PRAGUE, CZECH REPUBLIC
6. SLOVAK ACAD SCI, INST VIROL, BRATISLAVA, SLOVAKIA |
| Publisher: AMER SOC INVESTIGATIVE PATHOLOGY, INC, 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 |
| Subject Category: Pathology |
| IDS Number: PG110 |
| ISSN: 0002-9440 |
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