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| INDUCTION OF NUCLEAR FACTOR KAPPA-B AFTER LOW-DOSE IONIZING-RADIATION INVOLVES A REACTIVE OXYGEN INTERMEDIATE SIGNALING PATHWAY |
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| Author(s): MOHAN N, MELTZ ML |
| Source: RADIATION RESEARCH Volume: 140 Issue: 1 Pages: 97-104 Published: OCT 1994 |
| Times Cited: 86 References: 37 |
| Abstract: Reactive oxygen intermediates (ROIs) have been found to be the messengers in the activation of the kappa B transcription regulator in mitogen- or cytokine-stimulated cells, operating in conjunction with or independently of various other mechanisms; these include Ca++-dependent and PKC-dependent cytoplasmic signaling pathways, We have recently reported that low-dose ionizing radiation induces NF-kappa B in human lymphoblastoid 244B cells. Since ionizing radiation generates free radicals in cells, we have investigated whether the ROIs generated by ionizing radiation induce NF-kappa B activity, and also whether they do so by a similar mechanism as in cells treated with PMA or H2O2. The results not only confirm a previous observation from our laboratory that low-dose ionizing radiation (0.1-2.0 Gy) activates kappa B transcription factor transiently with a maximal induction at 0.5 Gy exposure, but also demonstrate mechanistically that the activation of NF-KB by low-dose ionizing radiation can be inhibited considerably by the antioxidant N-acetyl-L-cysteine, indicating that at least the major part of the activation process is mediated by ROIs. These findings support the idea that ROIs can regulate the kappa B elements which in turn can serve as response elements for oxidant stress. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV TEXAS, HLTH SCI CTR, DEPT RADIOL, DIV RADIAT ONCOL, SAN ANTONIO, TX 78284 USA |
| Publisher: RADIATION RESEARCH SOC, 2021 SPRING RD, STE 600, OAK BROOK, IL 60521 |
| Subject Category: Biology; Biophysics; Radiology, Nuclear Medicine & Medical Imaging |
| IDS Number: PK821 |
| ISSN: 0033-7587 |
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