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| IDENTIFICATION OF CRYPTIC SITES OF DNA-SEQUENCE AMPLIFICATION IN HUMAN BREAST-CANCER BY CHROMOSOME MICRODISSECTION |
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| Author(s): GUAN XY, MELTZER PS, DALTON WS, TRENT JM |
| Source: NATURE GENETICS Volume: 8 Issue: 2 Pages: 155-161 Published: OCT 1994 |
| Times Cited: 126 References: 30 |
| Abstract: We have performed microdissection of 16 putative homogeneously staining regions (hsrs) from nine different breast cancer cell lines in order to determine their chromosomal origin and composition. As expected, the most commonly amplified chromosomal band-region was 17q12 (containing ERBB2). However, regions not containing known oncogenes were also identified, including 13q31 (5/9 cases) and 20q12-13.2 (4/9 cases). The chromosomal composition of the integrated amplified DNA within each hsr was determined and in 13/16 cases (81%), hsrs were shown to be composed of two or more chromosomal regions. These studies shed light on the mechanism of formation of hsrs, and identify chromosomal regions likely to harbour genes amplified in breast cancer. |
| Document Type: Article |
| Language: English |
Addresses:
1. NIH, NATL CTR HUMAN GENOME RES, CANC GENET LAB, BETHESDA, MD 20892 USA
2. UNIV ARIZONA, ARIZONA COMPREHENS CANC CTR, TUCSON, AZ 85719 USA |
| Publisher: NATURE PUBLISHING CO, 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 |
| Subject Category: Genetics & Heredity |
| IDS Number: PL123 |
| ISSN: 1061-4036 |
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