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| SYNTHESIS OF PROTEINS BY NATIVE CHEMICAL LIGATION |
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| Author(s): DAWSON PE, MUIR TW, CLARKLEWIS I, KENT SBH |
| Source: SCIENCE Volume: 266 Issue: 5186 Pages: 776-779 Published: NOV 4 1994 |
| Times Cited: 1,007 References: 38 |
| Abstract: A simple technique has been devised that allows the direct synthesis of native backbone proteins of moderate size. Chemoselective reaction of two unprotected peptide segments gives an initial thioester-linked species. Spontaneous rearrangement of this transient intermediate yields a full-length product with a native peptide bond at the ligation site. The utility of native chemical ligation was demonstrated by the one-step preparation of a cytokine containing multiple disulfides. The polypeptide ligation product was folded and oxidized to form the native disulfide-containing protein molecule. Native chemical ligation is an important step toward the general application of chemistry to proteins. |
| Document Type: Article |
| Language: English |
Addresses:
1. SCRIPPS RES INST, LA JOLLA, CA 92037 USA
2. UNIV BRITISH COLUMBIA, BIOMED RES CTR, DEPT BIOCHEM, VANCOUVER V6T 1Z3, BC CANADA |
| Publisher: AMER ASSOC ADVANCEMENT SCIENCE, 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: PP753 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |