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| EXPRESSION OF ADHESION MOLECULES, PLATELET-ACTIVATING-FACTOR, AND CHEMOKINES BY KAPOSIS-SARCOMA CELLS |
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| Author(s): SCIACCA FL, STURZL M, BUSSOLINO F, SIRONI M, BRANDSTETTER H, ZIETZ C, ZHOU D, MATTEUCCI C, PERI G, SOZZANI S, BENELLI R, ARESE M, ALBINI A, COLOTTA F, MANTOVANI A |
| Source: JOURNAL OF IMMUNOLOGY Volume: 153 Issue: 10 Pages: 4816-4825 Published: NOV 15 1994 |
| Times Cited: 74 References: 63 |
| Abstract: The present study was designed to investigate whether cells cultured from Kaposi's sarcoma (KS), a vascular tumor with a prominent leukocyte infiltration, express molecules important for the recruitment and activation of leukocytes. KS cells expressed intercellular adhesion molecule-1, which was augmented by exposure to IL-1 beta or TNF-(a)lpha. Unlike endothelial cells, resting or cytokine-activated KS cells did not express appreciable levels of intercellular adhesion molecule-2, vascular cell adhesion molecule-1, and E-selectin on their surface. Weak expression of vascular cell adhesion molecule-1 mRNA was detectable by Northern blot analysis and, most clearly, by PCR analysis. Upon exposure to inflammatory cytokines, KS cells produced the attractant/activating lipid platelet-activating factor. KS cells expressed appreciable levels of the chemotactic cytokines, monocyte chemotactic protein-1 (MCP-1) and IL-8, as determined by Northern blot analysis, immunoassay, or bioassay. Chemokine production was augmented by IL-1 beta or TNF-alpha. MCP-1 expression was also detected in KS lesions by in situ hybridization. The set of molecules identified in the present study is probably important in determining the prominent leukocyte infiltration observed in KS. Tumor-associated leukocytes may amplify autocrine/paracrine circuits that sustain KS proliferation and contribute to recruitment of host vascular cells. |
| Document Type: Article |
| Language: English |
Addresses:
1. IST RIC FARMACOL MARIO NEGRI, I-20157 MILAN, ITALY
2. MAX PLANCK INST BIOCHEM, W-8033 MARTINSRIED, GERMANY
3. DEPT GENET MED BIOL & CHEM, TURIN, ITALY
4. UNIV MUNICH, INST PATHOL, W-8000 MUNICH, GERMANY
5. NATL INST CANC RES, GENOA, ITALY |
| Publisher: AMER ASSOC IMMUNOLOGISTS, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Immunology |
| IDS Number: PR222 |
| ISSN: 0022-1767 |
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