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| TYPE-C RETROVIRUS INACTIVATION BY HUMAN-COMPLEMENT IS DETERMINED BY BOTH THE VIRAL GENOME AND THE PRODUCER CELL |
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| Author(s): TAKEUCHI Y, COSSET FLC, LACHMANN PJ, OKADA H, WEISS RA, COLLINS MKL |
| Source: JOURNAL OF VIROLOGY Volume: 68 Issue: 12 Pages: 8001-8007 Published: DEC 1994 |
| Times Cited: 200 References: 31 |
| Abstract: The inactivation of type C retroviruses by human serum may be a considerable impediment to the use of retroviral vectors in vivo for gene therapy. Here we show that virus inactivation is dependent both on the virus and on the cell line used to produced the virus. All viruses produced from murine NIH 3T3 or dog Cf2ThS+L- cells are sensitive to human serum. In contrast, those produced from mink Mv-1-Lu and human HOS or TE671 cells are at least partially resistant, with the exception of murine leukemia viruses. In particular, the feline endogenous virus RD114 is completely resistant to a panel of eight human sera when produced from Mv-1-Lu or HOS cells. This differential resistance is controlled by the viral envelope proteins. Virus inactivation can be correlated with the ability of the producer cells to be lysed by human serum. Inactivation of sensitive viruses requires the classical pathway of complement but does not require virion lysis. |
| Document Type: Article |
| Language: English |
Addresses:
1. INST CANC RES, CHESTER BEATTY LABS, LONDON SW3 6JB, ENGLAND
2. UNIV CAMBRIDGE, CTR MRC, MOLEC IMMUNOPATHOL UNIT, CAMBRIDGE CB2 2QH, ENGLAND
3. GUNMA UNIV, SCH MED, DEPT HYG, MAEBASHI, GUMMA 371 JAPAN
4. NAGOYA CITY UNIV, SCH MED, DEPT BIOL MOLEC, NAGOYA, AICHI 467 JAPAN |
| Publisher: AMER SOC MICROBIOLOGY, 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 |
| Subject Category: Virology |
| IDS Number: PR432 |
| ISSN: 0022-538X |
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