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| SPECIFIC INCORPORATION OF CYCLOPHILIN-A INTO HIV-1 VIRIONS |
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| Author(s): FRANKE EK, YUAN HEH, LUBAN J |
| Source: NATURE Volume: 372 Issue: 6504 Pages: 359-362 Published: NOV 24 1994 |
| Times Cited: 397 References: 26 |
| Abstract: LITTLE is known about host factors necessary for retroviral virion assembly or uncoating. We have previously shown that the principal structural protein of the human immunodeficiency virus HIV-1, the Gag polyprotein, binds the cyclophilin peptidyl-prolyl isomerases(1); cyclophilins catalyse a rate-limiting step in protein folding(2) and protect cells from heat shock(3). Here we demonstrate that cyclophilin A is specifically incorporated into HIV-1 virions but not into virions of other primate immunodeficiency viruses. A proline-rich region conserved in all HIV-1 Gag polyproteins is required for cyclophilin A binding and incorporation. Disruption of a single proline blocks the Gag-cyclophilin interaction in vitro, prevents cyclophilin A incorporation into virions, and inhibits HIV-1 replication. Our results indicate that the interaction of Gag with cyclophilin A is necessary for the formation of infectious HIV-1 virions. |
| Document Type: Article |
| Language: English |
| Reprint Address: FRANKE, EK (reprint author), COLUMBIA UNIV, COLL PHYS & SURG, DEPT MED, 701 W 168TH ST, NEW YORK, NY 10032 USA |
| Publisher: MACMILLAN MAGAZINES LTD, PORTERS SOUTH, 4 CRINAN ST, LONDON, ENGLAND N1 9XW |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: PU287 |
| ISSN: 0028-0836 |
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