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| FIBRONECTIN ATTACHMENT ACTIVATES THE NF-KAPPA-B P50/P65 HETERODIMER IN FIBROBLASTS AND SMOOTH-MUSCLE CELLS |
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| Author(s): QWARNSTROM EE, OSTBERG CO, TURK GL, RICHARDSON CA, BOMSZTYK K |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 269 Issue: 49 Pages: 30765-30768 Published: DEC 9 1994 |
| Times Cited: 65 References: 33 |
| Abstract: The nuclear factor kappa B, NF-kappa B, is activated by numerous inflammatory mediators, regulating biological responses that in part are dependent on the extracellular matrix. Analyses of the influence of cell-matrix interaction on NF-kappa B activation revealed that attachment to fibronectin caused an increase in NF-kappa B activity corresponding to 3-fold the level in cells plated on bare plastic. This effect was dependent on the degree of attachment and appeared to involve both the RGD motif of the cell-binding domain, and the heparin-binding domain of fibronectin. Fibronectin attachment specifically caused activation of the p50/p65 heterodimeric form of NF-kappa B, resulting in an increase of this complex to a level similar to that induced by cytokine stimulation. These data show that cell attachment has a pronounced influence on the level of NF-kappa B activity, affecting specific dimeric complexes, and suggest this to be a pathway through which effects depending on cell-matrix interaction can regulate cell behavior and cytokine responses. |
| Document Type: Note |
| Language: English |
| Reprint Address: QWARNSTROM, EE (reprint author), UNIV WASHINGTON, DEPT PATHOL, SEATTLE, WA 98195 USA |
Addresses:
1. UNIV WASHINGTON, DEPT MED, SEATTLE, WA 98195 USA |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: PV510 |
| ISSN: 0021-9258 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |