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| OVEREXPRESSION OF CADHERINS AND UNDEREXPRESSION OF BETA-CATENIN INHIBIT DORSAL MESODERM INDUCTION IN EARLY XENOPUS EMBRYOS |
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| Author(s): HEASMAN J, CRAWFORD A, GOLDSTONE K, GARNERHAMRICK P, GUMBINER B, MCCREA P, KINTNER C, NORO CY, WYLIE C |
| Source: CELL Volume: 79 Issue: 5 Pages: 791-803 Published: DEC 2 1994 |
| Times Cited: 428 References: 54 |
| Abstract: The cadherin-catenin complex has an important role in cell-cell adhesion and may also function in signaling pathways. We report that overexpression of three cadherin types in Xenopus embryos causes them to develop with reduced dorsal axial structures. The same phenotype is produced in embryos that have been depleted of maternal beta-catenin protein by an antisense oligodeoxynucleotide complementary to beta-catenin mRNA. They show an inhibition in the expression of dorsal mesodermal markers MyoD and goosecoid, but not of ventral and general mesodermal markers. They lack notochords, somites, and neural tubes and are defective in dorsal mesodermal signaling in Nieuwkoop assays. The phenotype can be rescued by the injection of beta-catenin mRNA and not by the injection of Xwnt-8 mRNA. These results show that beta-catenin has an important role in dorsal mesoderm induction. They directly demonstrate the activity of a maternal mRNA in axis specification. |
| Document Type: Article |
| Language: English |
| Reprint Address: HEASMAN, J (reprint author), UNIV MINNESOTA, INST HUMAN GENET, MINNEAPOLIS, MN 55455 USA |
Addresses:
1. UNIV MINNESOTA, DEPT CELL BIOL & NEUROANAT, MINNEAPOLIS, MN 55455 USA
2. UNIV MINNESOTA, DEPT PEDIAT, MINNEAPOLIS, MN 55455 USA
3. WELLCOME CANC RES CTR INST, CAMBRIDGE CB2 1QR, ENGLAND
4. DEPT ZOOL, CAMBRIDGE CB2 1QR, ENGLAND
5. MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, NEW YORK, NY 10021 USA
6. SALK INST BIOL STUDIES, MOLEC NEUROBIOL LAB, SAN DIEGO, CA 92037 USA |
| Publisher: CELL PRESS, 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: PW054 |
| ISSN: 0092-8674 |
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