| OLIGONUCLEOTIDE-DIRECTED SELF-ASSEMBLY OF PROTEINS - SEMISYNTHETIC DNA STREPTAVIDIN HYBRID MOLECULES AS CONNECTORS FOR THE GENERATION OF MACROSCOPIC ARRAYS AND THE CONSTRUCTION OF SUPRAMOLECULAR BIOCONJUGATES |
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| Author(s): NIEMEYER CM, SANO T, SMITH CL, CANTOR CR |
| Source: NUCLEIC ACIDS RESEARCH Volume: 22 Issue: 25 Pages: 5530-5539 Published: DEC 25 1994 |
| Times Cited: 137 References: 24 |
| Abstract: Modified biomolecules were used for the non-covalent assembly of novel bioconjugates. Hybrid molecules were synthesized from short single-stranded DNA and streptavidin by chemical methods using a heterobi-specific crosslinker. The covalent attachment of an oligonucleotide moiety to streptavidin provides a specific recognition domain for a complementary nucleic acid sequence, in addition to the four native biotin-binding sites. These bispecific binding capabilities allow the hybrid molecules to serve as versatile connectors in a variety of applications. Bifunctional constructs have been prepared from two complementary hybrid molecules, each previously conjugated to biotinylated immunoglobulin G or alkaline phosphatase. The use of nucleic acid sequences as a template for the formation of an array of proteins is further demonstrated on two size scales. A macroscopic DNA array on a microtiter plate has been transformed into a comparable protein chip. A nano-scale array was made by hybridizing DNA-tagged proteins to specific positions along a RNA or DNA sequence. The generation of supramolecular bioconjugates was shown by quantitative measurements and gel-retardation assays. |
| Document Type: Article |
| Language: English |
Addresses:
1. BOSTON UNIV, CTR ADV BIOTECHNOL, BOSTON, MA 02215 USA |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: QC753 |
| ISSN: 0305-1048 |