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| MOLECULAR-CLONING OF HUMAN CDNA FOR CATHEPSIN-K - NOVEL CYSTEINE PROTEINASE PREDOMINANTLY EXPRESSED IN BONE |
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| Author(s): INAOKA T, BILBE G, ISHIBASHI O, TEZUKA K, KUMEGAWA M, KOKUBO T |
| Source: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Volume: 206 Issue: 1 Pages: 89-96 Published: JAN 5 1995 |
| Times Cited: 207 References: 23 |
| Abstract: We have previously cloned a rabbit cDNA clone (OC-2) from an osteoclast cDNA library by the differential screening. OC-2 was found to encode a novel cysteine proteinase, tentatively called cathepsin K, which is predominantly expressed in osteoclasts. By use of a rabbit OC-2 fragment as a probe, its human counterpart was cloned from a cDNA library of osteoarthritic hip bone. The cloned human cDNA (hOC-2) encoded a protein of 329 amino acid residues and its deduced amino acid sequence showed 94% homology to rabbit cathepsin K. Multiple alignment of amino acid sequences of human cathepsins B, H, L, S and K showed the highest homology of cathepsin K to cathepsin S 48%. Northern blot analysis showed that cathepsin K mRNA is expressed at high levels in some osteoarthritic hip bones and at a very high level in osteoclastoma compared to very low levels in other tissues. These results suggest that cathepsin K is closely involved in human osteoclastic bone resorption. (C) 1995 Academic Press, Inc. |
| Document Type: Article |
| Language: English |
Addresses:
1. CIBA GEIGY JAPAN LTD, INT RES LABS, TAKARAZUKA, HYOGO 665 JAPAN
2. CIBA GEIGY LTD, DEPT RES, DIV PHARMACEUT, CH-4002 BASEL, SWITZERLAND
3. MEIKAI UNIV, SCH DENT, DEPT ORAL ANAT, SAKADO, SAITAMA 35002 JAPAN |
| Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 |
| Subject Category: Biochemistry & Molecular Biology; Biophysics |
| IDS Number: QA375 |
| ISSN: 0006-291X |
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