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| GENETIC INTERACTION BETWEEN HOXB-5 AND HOXB-6 IS REVEALED BY NONALLELIC NONCOMPLEMENTATION |
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| Author(s): RANCOURT DE, TSUZUKI T, CAPECCHI MR |
| Source: GENES & DEVELOPMENT Volume: 9 Issue: 1 Pages: 108-122 Published: JAN 1 1995 |
| Times Cited: 161 References: 61 |
| Abstract: hoxb-5 and hoxb-6 are adjacent genes in the mouse HoxB locus and are members of the homeotic transcription factor complex that governs establishment of the mammalian body plan. To determine the roles of these genes during development, we generated mice with a targeted disruption in each gene. Three phenotypes affecting brachiocervicothoracic structures were found in the mutant mice. First, hoxb-5(-)homozygotes have a rostral shift of the shoulder girdle, analogous to what is seen in the human Sprengel anomaly. This suggests a role far hoxb-5 in specifying the position of limbs along the anteropasterior axis of the vertebrate body. Second, hoxb-6(-) homozygotes frequently have a missing first rib and a bifid second rib. The third phenotype, an anteriorizing homeotic transformation of the cervicothoracic vertebrae from C6 through T1, is common to both hoxb-5(-) and hoxb-6(-) homozygotes. Quite unexpectedly, hoxb-5, hoxb-6 transheterozygotes (hoxb-5(-)hoxb-6(+)/hoxb-5(+) hoxb-6(-)) also show the third phenotype. By this classical genetic complementation test, these two mutations appear as alleles of the same gene. This phenomenon is termed nonallelic noncomplementation and suggests that these two genes function together to specify this region of the mammalian vertebral column. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV UTAH, SCH MED, DEPT HUMAN GENET, HOWARD HUGHES MED INST, SALT LAKE CITY, UT 84112 USA |
| Publisher: COLD SPRING HARBOR LAB PRESS, 1 BUNGTOWN RD, PLAINVIEW, NY 11724 |
| Subject Category: Cell Biology; Developmental Biology; Genetics & Heredity |
| IDS Number: QB810 |
| ISSN: 0890-9369 |
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