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| DISSECTING RNA-PROTEIN INTERACTIONS - RNA-RNA RECOGNITION BY ROP |
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| Author(s): PREDKI PF, NAYAK LM, GOTTLIEB MBC, REGAN L |
| Source: CELL Volume: 80 Issue: 1 Pages: 41-50 Published: JAN 13 1995 |
| Times Cited: 81 References: 45 |
| Abstract: The ColE1 plasmid of E. coli encodes a small RNA-binding protein, Rop, which is involved in the regulation of plasmid copy number, Rop, a 4-helix bundle protein, facilitates sense-antisense RNA pairing by binding to the transiently formed hairpin pairs of RNA I and the complementary RNA II. We have identified the residues of Rop that are involved in RNA recognition. The residues form a narrow stripe down one face of the bundle and are symmetrically arranged, with recognition centered about two phenylalanine residues. Our results suggest that these phenylalanine residues interact with the loop region of the hairpin pair, with additional interactions between eight polar residues and the phosphate backbone. By modifying the identity of residue 14, we have created a variant of Rop that displays altered RNA binding specificity. The results of our studies allow us to present a detailed picture of RNA-protein recognition in a novel model system. |
| Document Type: Article |
| Language: English |
Addresses:
1. YALE UNIV, DEPT MOLEC BIOPHYS & BIOCHEM, NEW HAVEN, CT 06511 USA |
| Publisher: CELL PRESS, 50 CHURCH ST CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: QB910 |
| ISSN: 0092-8674 |
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