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| GATING OF THE VOLTAGE-DEPENDENT CHLORIDE CHANNEL CIC-O BY THE PERMEANT ANION |
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| Author(s): PUSCH M, LUDEWIG U, REHFELDT A, JENTSCH TJ |
| Source: NATURE Volume: 373 Issue: 6514 Pages: 527-531 Published: FEB 9 1995 |
| Times Cited: 239 References: 29 |
| Abstract: CHLORIDE channels of the ClC family are important for the control of membrane excitability(1-3), cell volume regulation(4,5), and possibly transepithelial transport lacking the typical voltage-sensor found in cation gating of ClC channels is clearly voltage-dependent. For the prototype Torpedo channel ClC-0 (refs 11-15) we now show that channel opening is strongly facilitated by external chloride. Other less permeable anions can substitute for chloride with less efficiency. ClC-0 conductance shows an anomalous mole fraction behaviour with Cl-/NO3- mixtures, suggesting a multi-ion pore. Gating shows a similar anomalous behaviour, tightly linking permeation to gating. Eliminating a positive charge at the cytoplasmic end of domain D12 changes kinetics, concentration dependence and halide selectivity of gating, and alters pore properties such as ion selectivity, single-channel conductance and rectification. Taken together, our results strongly suggest that in these channels voltage-dependent gating is conferred by the permeating ion itself, acting as the gating charge. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV HAMBURG, CTR MOLEC NEUROBIOL, D-20246 HAMBURG, GERMANY |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: QF724 |
| ISSN: 0028-0836 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |