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| STRUCTURALLY VARIANT CLASSES OF PILUS APPENDAGE FIBERS COEXPRESSED FROM BURKHOLDERIA (PSEUDOMONAS) CEPACIA |
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| Author(s): GOLDSTEIN R, SUN L, JIANG RZ, SAJJAN U, FORSTNER JF, CAMPANELLI C |
| Source: JOURNAL OF BACTERIOLOGY Volume: 177 Issue: 4 Pages: 1039-1052 Published: FEB 1995 |
| Times Cited: 38 References: 60 |
| Abstract: One or more of five morphologically distinct classes of appendage pili were determined to be peritrichously expressed by Burkholderia (formerly Pseudomonas) cepacia isolated from disparate sources. B. cepacia-encoded cblA pilin gene hybridization-based analysis revealed that one associated class, cable (Cbl) adhesin type IIB. cepacia pili, correlates with epidemically transmitted strains from a single cystic fibrosis (CF) center. When only phenotypic assays were available, correlations between the source and the pilus type were nonetheless observed: filamentous (Fil) type IIIB. cepacia pili correlated with CF-associated nonepidemic isolates, spine (Spn) type IVB. cepacia pili correlated with clinical (non-CF) isolates, and spike (Spk) type V-B. cepacia pili correlated with environmental isolates. Further, Cbl, Fil, or Spk pili typically appear as an internal framework for constitutively coexpressed, peritrichously arranged dense mats of fine, curly mesh (Msh) type I-B. cepacia pili. Constitutive coexpression of dense mats of Msh type I-B. cepacia pili in association with a labyrinth of either Cbl, Fil, or Spk pili suggests possible cooperative pilus interactions mediating adhesion-based colonization in the differing environments from which the strains were isolated. Despite such correlations, phylogenetic analyses indicate that with the exception of the epidemically transmitted clusters of isolates, the remaining B. cepacia strains from the other three sources exhibited an equal degree of genetic relatedness independent of origin. As previously found for Escherichia coli, this discrepancy could be accounted for by selection-driven, in vivo horizontal transfer events between distantly related members of the species B. cepacia, leading to the genetic acquisition of environmentally appropriate adhesion-based colonization pilus operons. |
| Document Type: Article |
| Language: English |
| Reprint Address: GOLDSTEIN, R (reprint author), BOSTON UNIV, SCH MED, MAXWELL FINLAND LAB INFECT DIS, MOLEC GENET SECT, 774 ALBANY ST, BOSTON, MA 02118 USA |
Addresses:
1. BOSTON CITY HOSP, BOSTON, MA 02118 USA
2. HOSP SICK CHILDREN, RES INST, DIV BIOCHEM, TORONTO, ON M5G 1X8 CANADA |
| Publisher: AMER SOC MICROBIOLOGY, 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 |
| Subject Category: Microbiology |
| IDS Number: QG250 |
| ISSN: 0021-9193 |
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