| | |  | | | | Record from Web of Science® | | | | | | |
| IMPAIRED TUBULOGENESIS OF CYST-DERIVED CELLS FROM AUTOSOMAL-DOMINANT POLYCYSTIC KIDNEYS |
|
|
| Author(s): CARONE FA, NAKAMURA S, BACALLAO R, NELSON WJ, KHOKHA M, KANWAR YS |
| Source: KIDNEY INTERNATIONAL Volume: 47 Issue: 3 Pages: 861-868 Published: MAR 1995 |
| Times Cited: 23 References: 31 |
| Abstract: Under appropriate growth factor or hormonal influence. renal epithelial cells cultured in collagen gels form branching tubular elements, reminiscent of metanephric tubulogenesis. This study evaluates the phenotypic characteristics of normal human renal epithelial cells (NK) and epithelial cells from cysts of autosomal dominant polycystic kidneys (ADPKD) gown in collagen gels under the influence of the growth factors (GFs) epidermal (EGF), transforming (TGF-alpha), hepatocyte (HGF) and fibroblast (FGF). All GFs induced cell proliferation with the formation of cell aggregates in both group of cells, however, NK cells exhibited proliferation at a much higher rate compared to ADPKD. All GFs induced formation of branching tubular elements with cell-polarity characteristics in NK cells. Such organized tubular elements were essentially absent in ADPKD cell cultures. Both NK and ADPKD cells expressed cell adhesion and matrix macromolecules. Expression of heparan sulfate-proteoglycan was diminished but enhanced for fibronectin in ADPKD cells. Receptor expression for EGF and FGF was similar. These findings indicate an impairment in tubulogenesis of ADPKD cells, perhaps related to the aberrant morphogenetic cell aggregation. Alternatively, this differentiation arrest may relate to abnormal biosynthesis of secretory matrix glycoproteins rather than those expressed on the plasmalemma. |
| Document Type: Article |
| Language: English |
| Reprint Address: CARONE, FA (reprint author), NORTHWESTERN UNIV, SCH MED, DEPT PATHOL, CHICAGO, IL 60611 USA |
Addresses:
1. NORTHWESTERN UNIV, SCH MED, DEPT MED & CELL, CHICAGO, IL 60611 USA
2. NORTHWESTERN UNIV, SCH MED, DEPT MOLEC & STRUCT BIOL, CHICAGO, IL 60611 USA
3. STANFORD UNIV, SCH MED, DEPT CELLULAR & MOLEC PHYSIOL, STANFORD, CA USA |
| Publisher: BLACKWELL SCIENCE PUBL INC CAMBRIDGE, 238 MAIN ST, CAMBRIDGE, MA 02142 |
| Subject Category: Urology & Nephrology |
| IDS Number: QH038 |
| ISSN: 0085-2538 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |