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| HISTONE H3 AND H4 N-TERMINI INTERACT WITH SIR3 AND SIR4 PROTEINS - A MOLECULAR-MODEL FOR THE FORMATION OF HETEROCHROMATIN IN YEAST |
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| Author(s): HECHT A, LAROCHE T, STRAHLBOLSINGER S, GASSER SM, GRUNSTEIN M |
| Source: CELL Volume: 80 Issue: 4 Pages: 583-592 Published: FEB 24 1995 |
| Times Cited: 545 References: 43 |
| Abstract: The silent mating loci and chromosomal regions adjacent to telomeres of S. cerevisiae have features similar to heterochromatin of more complex eukaryotes. Transcriptional repression at these sites depends on the silent information regulators SIR3 and SIR4 as well as histones H3 and H4. We show here that the SIR3 and SIR4 proteins interact with specific silencing domains of the H3 and H4 N-termini in vitro. Certain mutations in these factors, which affect their silencing functions in vivo, also disrupt their interactions in vitro. Immunofluorescence studies with antibodies against RAP1 and SIR3 demonstrate that the H3 and H4 N-termini are required for the association of SIR3 with telomeric chromatin and the perinuclear positioning of yeast telomeres. Based on these interactions, we propose a model for heterochromatin-mediated transcriptional silencing in yeast, which may serve as a paradigm for other eukaryotic organisms as well. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV CALIF LOS ANGELES, SCH MED, DEPT BIOL CHEM, LOS ANGELES, CA 90095 USA
2. UNIV CALIF LOS ANGELES, INST MOLEC BIOL, LOS ANGELES, CA 90095 USA
3. SWISS INST EXPTL CANC RES, CH-1066 EPALINGES, SWITZERLAND |
| Publisher: CELL PRESS, 50 CHURCH ST CIRCULATION DEPT, CAMBRIDGE, MA 02138 |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: QJ602 |
| ISSN: 0092-8674 |
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