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| INTERACTION OF THYROID-HORMONE RECEPTOR WITH A CONSERVED TRANSCRIPTIONAL MEDIATOR |
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| Author(s): LEE JW, RYAN F, SWAFFIELD JC, JOHNSTON SA, MOORE DD |
| Source: NATURE Volume: 374 Issue: 6517 Pages: 91-94 Published: MAR 2 1995 |
| Times Cited: 371 References: 20 |
| Abstract: THE thyroid-hormone receptors are hormone-dependent transcription factors that control expression of many target genes(1,2). This regulation is presumably a consequence of hormone-dependent contacts between the receptors and the basal transcription machinery(3). We used the yeast two-hybrid system(4,5) to identify a candidate human transcriptional mediator that interacts with both the thyroid-hormone receptor and the retinoid-X receptor in a ligand-dependent fashion. This protein, Trip1 (for thyroid-hormone-receptor Interacting protein), shares striking sequence conservation with the yeast transcriptional mediator Sug1 (refs 6, 7). Here we show that Trip1 can functionally substitute for Sug1 in yeast, and that both proteins interact in vitro with the thyroid-hormone receptor, acid with the transcriptional activation domains of yeast GAL4 and of herpes virus VP16. |
| Document Type: Article |
| Language: English |
| Reprint Address: LEE, JW (reprint author), MASSACHUSETTS GEN HOSP, DEPT MOLEC BIOL, WELLMAN 9, BOSTON, MA 02114 USA |
Addresses:
1. UNIV TEXAS, SW MED CTR, DEPT MED, DALLAS, TX 75235 USA
2. UNIV TEXAS, SW MED CTR, DEPT BIOCHEM, DALLAS, TX 75235 USA |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: QK079 |
| ISSN: 0028-0836 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |