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| A 3'-UNTRANSLATED REGION OF CATALASE MESSENGER-RNA COMPOSED OF A STEM-LOOP AND DINUCLEOTIDE REPEAT ELEMENTS BINDS A 69-KDA REDOX-SENSITIVE PROTEIN |
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| Author(s): CLERCH LB |
| Source: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS Volume: 317 Issue: 1 Pages: 267-274 Published: FEB 20 1995 |
| Times Cited: 37 References: 14 |
| Abstract: Rat lung extract contains protein that forms redox-sensitive, specific complexes with a 1130-base catalase cRNA (J. Biol. Chem. 267, 2853-2855, 1992). The present paper reports studies aimed at delimiting the site of protein binding on the RNA and characterizing the protein. A 240-base sequence was identified as the 3' untranslated region of catalase mRNA that binds lung protein in a redox-sensitive manner. Two elements within this 240-base region bind protein; one is a 36-base element that has a computer-predicted stem-loop secondary structure and the other is a CA dinucleotide repeat. Competition studies indicate that both elements are required for specific binding. Cross-competition experiments demonstrated that catalase RNA-binding protein (CAT-BP) is not the iron-responsive element-binding protein. Ultraviolet light-induced cross-linking and two-dimensional electrophoresis showed that CAT-BP has an apparent molecular mass of 69 kDa and appears to be composed of four isoforms. Competition studies indicate the stem-loop cis element is directly involved in binding CAT-BP. In addition to rat, the 69-kDa catalase RNA-binding protein is present in mouse and human fibroblast cell lines. (C) 1995 Academic Press, Inc. |
| Document Type: Article |
| Language: English |
| Reprint Address: CLERCH, LB (reprint author), GEORGETOWN UNIV, MED CTR, LUNG BIOL LAB, PRECLIN SCI BLDG, GM12, 3900 RESERVOIR RD, WASHINGTON, DC 20007 USA |
| Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 |
| Subject Category: Biochemistry & Molecular Biology; Biophysics |
| IDS Number: QK397 |
| ISSN: 0003-9861 |
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