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| LAMIN PROTEINS FORM AN INTERNAL NUCLEOSKELETON AS WELL AS A PERIPHERAL LAMINA IN HUMAN-CELLS |
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| Author(s): HOZAK P, SASSEVILLE AMJ, RAYMOND Y, COOK PR |
| Source: JOURNAL OF CELL SCIENCE Volume: 108 Pages: 635-644 Part: Part 2 Published: FEB 1995 |
| Times Cited: 202 References: 62 |
| Abstract: The nuclear lamina forms a protein mesh that underlies the nuclear membrane, In most mammalian cells it contains the intermediate filament proteins, lamins A, B and C. As their name indicates, lamins are generally thought to be confined to the nuclear periphery, We now show that they also form part of a diffuse skeleton that ramifies throughout the interior of the nucleus, Unlike their peripheral counterparts, these internal lamins are buried in dense chromatin and so are inaccessible to antibodies, but accessibility can be increased by removing chromatin, Knobs and nodes on an internal skeleton can then be immunolabelled using fluorescein- or gold-conjugated anti-lamin A antibodies, These results suggest that the lamins are misnamed as they are also found internally. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV OXFORD, SIR WILLIAM DUNN SCH PATHOL, CRC, NUCL STUCT & FUNCT RES GRP, OXFORD OX1 3RE, ENGLAND
2. ACAD SCI CZECH REPUBL, INST EXPTL MED, CELL ULTRASTRUCT LAB, CR-14220 PRAGUE 4, CZECH REPUBLIC
3. UNIV MONTREAL, INST CANC MONTREAL, CTR RECH LOUIS CHARLES SIMARD, MONTREAL, PQ H2L 4M1 CANADA |
| Publisher: COMPANY OF BIOLOGISTS LTD, BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL |
| Subject Category: Cell Biology |
| IDS Number: QK898 |
| ISSN: 0021-9533 |
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