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| SEVERE COMBINED IMMUNODEFICIENCY MOUSE AND HUMAN PSORIATIC SKIN CHIMERAS - VALIDATION OF A NEW ANIMAL-MODEL |
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| Author(s): NICKOLOFF BJ, KUNKEL SL, BURDICK M, STRIETER RM |
| Source: AMERICAN JOURNAL OF PATHOLOGY Volume: 146 Issue: 3 Pages: 580-588 Published: MAR 1995 |
| Times Cited: 80 References: 35 |
| Abstract: Research into the cause and pathophysiological mechanisms underlying expression of psoriatic skin lesions has been hampered by lack of an appropriate animal model for this common and enigmatic cutaneous disease These studies characterize normal skin, pre-psoriatic skin, and psoriatic plaque skill samples transplanted onto severe combined immunodeficiency mice, In this report we document that 1), normal, prepsoriatic, and psoriatic plaque keratome skin samples can be transplanted onto severe combined immunodeficiency mice reliably with high rates of graft survival (>85%) and with reproducible changes consistently observed over prolonged periods of engraftment; 2), after transplantation, by clinical assessment and routine light microscopy, normal skin remained essentially normal whereas pre-psoriatic skin became thicker, and psoriatic plaque skin retained its characteristic plaque-type elevation and scale; 3), by using a panel of antibodies and immuno-histochemical analysis, the overall phenotype of human cell types (including immunocytes) that persisted in the transplanted skin was remarkably similar to the immmunophenotype of pretransplanted skirt samples; 4), clearly recognized interface zones between human and murine skin within the epidermal and dermal compartments could be identified by routine microscopy and immunostaining, with focal areas of chimerism; and 5), elevated interleukin 8 cytokine levels were present in transplanted pre-psoriatic and psoriatic plaque skin samples, Ne conclude that there are many similarities between pre- and post-transplanted human samples of normal and psoriatic skin that are grafted onto severe combined immunodeficiency mice, Thus, Me propose that this new animal model is appropriate for additional mechanistic-type studies designed to reveal the underlying genetic/etiological abnormality, as well as better illuminate the pathophysiological basis,for this important skin disease. |
| Document Type: Note |
| Language: English |
| Reprint Address: NICKOLOFF, BJ (reprint author), UNIV MICHIGAN, SCH MED, DEPT PATHOL, M4232 MED SCI I, 1301 E CATHERINE ST, ANN ARBOR, MI 48109 USA |
Addresses:
1. UNIV MICHIGAN, SCH MED, DEPT INTERNAL MED, DIV PULM & CRIT CARE MED, ANN ARBOR, MI USA |
| Publisher: AMER SOC INVESTIGATIVE PATHOLOGY, INC, 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 |
| Subject Category: Pathology |
| IDS Number: QL395 |
| ISSN: 0002-9440 |
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