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| CPG MOTIFS IN BACTERIAL-DNA TRIGGER DIRECT B-CELL ACTIVATION |
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| Author(s): KRIEG AM, YI AK, MATSON S, WALDSCHMIDT TJ, BISHOP GA, TEASDALE R, KORETZKY GA, KLINMAN DM |
| Source: NATURE Volume: 374 Issue: 6522 Pages: 546-549 Published: APR 6 1995 |
| Times Cited: 1,930 References: 29 |
| Abstract: UNMETHYLATED CpG dinucleotides are more frequent in the genomes of bacteria and viruses than of vertebrates. We report here that bacterial DNA and synthetic oligodeoxynucleotides containing unmethylated CPG dinucleotides induce murine B cells to proliferate and secrete immunoglobulin in vitro and in vivo. This activation is enhanced by simultaneous signals delivered through the antigen receptor. Optimal B-cell activation requires a DNA motif in which an unmethylated CpG dinucleotide is flanked by two 5' purines and two 3' py,rimidines. Oligodeoxynucleotides containing this CpG motif induce more than 95% of all spleen B cells to enter the cell cycle. These data suggest a possible evolutionary link between immune defence based on the recognition of microbial DNA and the phenomenon of CpG suppression' in vertebrates. The potent immune activation by CpG oligonucleotides has implications for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants. |
| Document Type: Article |
| Language: English |
| Reprint Address: KRIEG, AM (reprint author), UNIV IOWA, COLL MED, DEPT INTERNAL MED, IOWA CITY, IA 52242 USA |
Addresses:
1. UNIV IOWA, COLL MED, DEPT PATHOL, IOWA CITY, IA 52242 USA
2. UNIV IOWA, COLL MED, DEPT MICROBIOL, IOWA CITY, IA 52242 USA
3. UNIV IOWA, COLL MED, DEPT PHYSIOL, IOWA CITY, IA 52242 USA
4. VET AFFAIRS MED CTR, IOWA CITY, IA 52246 USA
5. US FDA, CTR BIOL EVALUAT & RES, RETROVIRAL IMMUNOL SECT, BETHESDA, MD 20892 USA |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: QR069 |
| ISSN: 0028-0836 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |