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| FAST AXONAL-TRANSPORT OF KINESIN IN THE RAT VISUAL-SYSTEM - FUNCTIONALITY OF KINESIN HEAVY-CHAIN ISOFORMS |
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| Author(s): ELLURU RG, BLOOM GS, BRADY ST |
| Source: MOLECULAR BIOLOGY OF THE CELL Volume: 6 Issue: 1 Pages: 21-40 Published: JAN 1995 |
| Times Cited: 75 References: 67 |
| Abstract: The mechanochemical ATPase kinesin is thought to move membrane-bounded organelles along microtubules in fast axonal transport. However, fast transport includes several classes of organelles moving at rates that differ by an order of magnitude. Further, the fact that cytoplasmic forms of kinesin exist suggests that kinesins might move cytoplasmic structures such as the cytoskeleton. To define cellular roles for kinesin, the axonal transport of kinesin was characterized. Retinal proteins were pulse-labeled, and movement of radiolabeled kinesin through optic nerve and tract into the terminals was monitored by immunoprecipitation. Heavy and light chains of kinesin appeared in nerve and tract at times consistent with fast transport. Little or no kinesin moved with slow axonal transport indicating that effectively all axonal kinesin is associated with membranous organelles. Both kinesin heavy chain molecular weight variants of 130,000 and 124,000 M, (KHC-A and KHC-B) moved in fast anterograde transport, but KHC-A moved at 5-6 times the rate of KHC-B. KHC-A cotransported with the synaptic vesicle marker synaptophysin, while a portion of KHC-8 cotransportee with the mitochondrial marker hexokinase. These results suggest that KHC-A is enriched on small tubulovesicular structures like synaptic vesicles and that at least one form of KHC-8 is predominantly on mitochondria. Biochemical specialization may target kinesins to appropriate organelles and facilitate differential regulation of transport. |
| Document Type: Article |
| Language: English |
| Reprint Address: ELLURU, RG (reprint author), UNIV TEXAS, SW MED CTR, DEPT CELL BIOL & NEUROSCI, DALLAS, TX 75235 USA |
| Publisher: AMER SOC CELL BIOL, PUBL OFFICE 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Cell Biology |
| IDS Number: QT650 |
| ISSN: 1059-1524 |
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