| | |  | | | | Record from Web of Science® | | | | | | |
| GENERATION AND ASSEMBLY OF SECRETORY ANTIBODIES IN PLANTS |
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| Author(s): MA JKC, HIATT A, HEIN M, VINE ND, WANG F, STABILA P, VANDOLLEWEERD C, MOSTOV K, LEHNER T |
| Source: SCIENCE Volume: 268 Issue: 5211 Pages: 716-719 Published: MAY 5 1995 |
| Times Cited: 296 References: 24 |
| Abstract: Four transgenic Nicotiana tabacum plants were generated that expressed a murine monoclonal antibody kappa chain, a hybrid immunoglobulin A-G heavy chain, a murine joining chain, and a rabbit secretory component, respectively. Successive sexual crosses between these plants and filial recombinants resulted in plants that expressed all four protein chains simultaneously. These chains were assembled into a functional, high molecular weight secretory immunoglobulin that recognized the native streptococcal antigen I/II cell surface adhesion molecule. In plants, single cells are able to assemble secretory antibodies, whereas two different cell types are required in mammals. Transgenic plants may be suitable for large-scale production of recombinant secretory immunoglobulin A for passive mucosal immunotherapy. Plant cells also possess the requisite mechanisms for assembly and expression of other complex recombinant protein molecules. |
| Document Type: Article |
| Language: English |
| Reprint Address: MA, JKC (reprint author), UNITED MED & DENT SCH, GUYS HOSP, DEPT IMMUNOL, 28TH FLOOR, GUYS TOWER, LONDON SE1 9RT, ENGLAND |
Addresses:
1. PLANET BIOTECHNOL, SAN DIEGO, CA 92121 USA
2. Scripps Res Inst, DEPT CELL BIOL, DIV PLANT BIOL, LA JOLLA, CA 92037 USA
3. UNIV CALIF SAN FRANCISCO, SCH MED, SAN FRANCISCO, CA 94143 USA |
| Publisher: AMER ASSOC ADVAN SCIENCE, 1333 H ST NW, WASHINGTON, DC 20005 |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: QW603 |
| ISSN: 0036-8075 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |