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| STIMULATION OF SULFATED GLYCOSAMINOGLYCAN AND DECORIN PRODUCTION IN ADULT DERMAL FIBROBLASTS BY RECOMBINANT HUMAN INTERLEUKIN-4 |
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| Author(s): WEGROWSKI Y, PALTOT V, GILLERY P, KALIS B, RANDOUX A, MAQUART FX |
| Source: BIOCHEMICAL JOURNAL Volume: 307 Pages: 673-678 Part: Part 3 Published: MAY 1 1995 |
| Times Cited: 40 References: 46 |
| Abstract: Interleukin-4 (IL-4) is a pleiotropic cytokine expressed by inflammatory cells. Previous work from our laboratory has shown that it stimulates collagen synthesis in fibroblasts. Here we report the effects of recombinant human IL-4 on glycosaminoglycan (GAG) and proteoglycan synthesis in normal dermal fibroblasts from adult donors. IL-4 (10 and 100 units/ml) induced a dose-dependent increase of [H-3]glucosamine and [S-35]sulphate incorporation into total GAGs. The analysis of the different GAG fractions indicated the enhanced synthesis of dermatan/chondroitin sulphates. IL-4 had no effect on hyaluronan synthesis. The increase of sulphated GAG synthesis was correlated with an increase of proteoglycans in the culture medium. Decorin was identified as the major chondroitin/dermatan sulphate-containing proteoglycan in the culture medium of fibroblasts. Its synthesis was strongly stimulated by IL-4. Both the core-protein synthesis and mRNA expression were enhanced, indicating that the cytokine acted, at least in part, at the pre-translational level. These results indicate that IL-4 is able to modulate not only collagen, but also proteoglycan, production by human fibroblasts. Their implications in physiopathological processes such as wound healing or fibrosis is suggested. |
| Document Type: Article |
| Language: English |
Addresses:
1. FAC MED REIMS, BIOCHIM LAB, CNRS, ER 084, F-51095 REIMS, FRANCE
2. FAC MED REIMS, RECH BIOMED DERMATOL LAB, F-51095 REIMS, FRANCE |
| Publisher: PORTLAND PRESS, 59 PORTLAND PLACE, LONDON W1N 3AJ, ENGLAND |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: QW667 |
| ISSN: 0264-6021 |
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