| IDENTIFICATION AND CHARACTERIZATION OF USS1P (SDB23P) - A NOVEL U6 SNRNA-ASSOCIATED PROTEIN WITH SIGNIFICANT SIMILARITY TO CORE PROTEINS OF SMALL NUCLEAR RIBONUCLEOPROTEINS |
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| Author(s): COOPER M, JOHNSTON LH, BEGGS JD |
| Source: EMBO JOURNAL Volume: 14 Issue: 9 Pages: 2066-2075 Published: MAY 1 1995 |
| Times Cited: 102 References: 55 |
| Abstract: The SDB23 gene of Saccharomyces cerevisiae was isolated in a search for high copy-number suppressors of mutations in a cell cycle gene, DBF2. SDB23 encodes a 21 276 Da protein with significant sequence similarity to characterized mammalian snRNP core proteins. Examination of multiple sequence alignments of snRNP core proteins with Sdb23p indicates that all of these proteins share a number of highly conserved residues, and identifies a novel motif for snRNP core proteins. Sdb23p is essential for cell viability and is required for nuclear pre-mRNA splicing both in vivo and in vitro. Extracts prepared from Sdb23p-depleted cells are unable to support splicing and have vastly reduced levels of U6 snRNA. The stability of U1, U2, U4 and U5 spliceosomal snRNAs is not affected by the loss of Sdb23p. Antibodies raised against Sdb23p strongly co-immunoprecipitate free U6 snRNA and U4/U6 base-paired snRNAs. These results establish that SDB23 encodes a novel U6 snRNA-associated protein that is essential for the stability of U6 snRNA. We therefore propose the more logical name USS1 (U-Six SnRNP) for this gene. |
| Document Type: Article |
| Language: English |
Addresses:
1. UNIV EDINBURGH, INST CELL & MOLEC BIOL, EDINBURGH EH9 3JR, MIDLOTHIAN SCOTLAND
2. NATL INST MED RES, YEAST GENET LAB, LONDON NW7 1AA, ENGLAND |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: QX744 |
| ISSN: 0261-4189 |