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| REPRESSOR ACTIVITY OF CCAAT DISPLACEMENT PROTEIN IN HL-60 MYELOID-LEUKEMIA CELLS |
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| Author(s): LIEVENS PMJ, DONADY JJ, TUFARELLI C, NEUFELD EJ |
| Source: JOURNAL OF BIOLOGICAL CHEMISTRY Volume: 270 Issue: 21 Pages: 12745-12750 Published: MAY 26 1995 |
| Times Cited: 92 References: 29 |
| Abstract: CCAAT displacement protein (CDP)/cut is implicated in several systems as a transcriptional repressor of developmentally regulated genes. In myeloid leukemia cells, CDP/cut binding activity as assayed on the promoter of the phagocyte-specific cytochrome heavy chain gene gp91-phox varies inversely with expression of gp91-phox mRNA. We used two approaches to ascertain whether CDP/cut serves as a repressor of gp91-phox gene expression, First, we used transient transfection assays in 3T3 cells to demonstrate that the CDP/cut binding site from the gp91-phox promoter acts as a negative regulatory element in artificial promoter constructs, Second, we isolated a stable transformant of HL-60 myeloid cells constitutively expressing transfected CDP/cut cDNA, Stable transformants carrying expression vector alone or expressing CDP/cut mRNA were induced to differentiate along the macrophage lineage with phorbol ester or along the neutrophil lineage with dimethyl sulfoxide or retinoic acid/dimethylformamide, Northern blot analysis was used to assess induction of mRNAs encoding gp91-phox, and the myeloid oxidase cytosolic components, p47 and p67, In the stable transformant expressing transfected CDP/cut cDNA, gp91-phox induction was selectively reduced, whereas morphologic differentiation and induction of mRNA for myeloid oxidase components p47 and p67 were unaffected, These data provide persuasive evidence that CDP/cut acts to repress the gp91-phox gene. |
| Document Type: Article |
| Language: English |
Addresses:
1. CHILDRENS HOSP, DANA FARBER CANC INST, DIV PEDIAT HEMATOL ONCOL, BOSTON, MA 02115 USA
2. HARVARD UNIV, SCH MED, BOSTON, MA 02115 USA |
| Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: QZ711 |
| ISSN: 0021-9258 |
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