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| HUMAN SKIN MAST-CELLS EXPRESS FUNCTIONAL BETA(1) INTEGRINS THAT MEDIATE ADHESION TO EXTRACELLULAR-MATRIX PROTEINS |
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| Author(s): COLUMBO M, BOCHNER BS, MARONE G |
| Source: JOURNAL OF IMMUNOLOGY Volume: 154 Issue: 11 Pages: 6058-6064 Published: JUN 1 1995 |
| Times Cited: 46 References: 42 |
| Abstract: We have evaluated the adhesion of human cutaneous mast cells to several components of the extracellular matrix (plasma fibronectin, laminin, collagen type I and IV) and studied whether these cells express the beta(1) integrins potentially involved in the adhesion to these proteins. Human skin mast cells (5.1 +/- 1.5% pure) spontaneously adhered to fibronectin and laminin (0.1 to 10 mu g/ml) immobilized on plastic surfaces (e.g., 14 +/- 7.2% and 14 +/- 4.4% adhesion at 10 mu g/ml, respectively). Similar results were obtained with a 90% pure mast cell preparation. In contrast, cutaneous mast cells did not adhere to collagen type I (1.6 +/- 0.5% adhesion) or type IV (1.2 +/- 0.8% adhesion). Control adhesion in BSA-coated wells was <5%. Mast cell adhesion to fibronectin was optimal after an incubation period of 60 to 90 min (t(1/2) = 28.2 1 6.2 min), whereas adhesion to laminin was faster (t(1/2) = 10.1 +/- 1.2 min), being nearly optimal after a 15-min incubation period. Human skin mast cell adhesion to fibronectin and laminin was found to be dependent on the presence of divalent cations in the extracellular medium. Dual-color immunofluorescence and flow cytometry were used to evaluate whether human skin mast cells (51.3 +/- 3.9% pure) express beta(1) integrins that may mediate cell adhesion to extracellular matrix proteins. These mast cells were found to express VLA (very late Ag)-3 (75.3 +/- 35.6 specific fluorescence intensity) and, to lesser degree, VLA-4 and VLA-5 receptors (8.0 +/- 2.5 and 6.9 +/- 3.2 specific fluorescence intensity, respectively). In contrast, VLA-1, VLA-2, and VLA-6 integrins were not expressed significantly. mAb to VLA-3, VLA-4, and VLA-5 each inhibited by 70% skin mast cell adhesion to fibronectin. mAb to VLA-3 nearly abolished mast cells adhesion to laminin, whereas anti-VLA-4 and anti-VLA-5 were ineffective. Finally, immunosuppressant cyclosporin A (100 nM) and FK-506 (10 nM) significantly inhibited mast cell adhesion to both fibronectin and laminin (p < 0.05). Our data demonstrate that human skin mast cells spontaneously adhere to fibronectin and laminin, and that this adhesion is mediated by VLA-3, VLA-4, and/or VLA-5 integrins on these cells. Interactions between these beta(1) integrins and extracellular matrix proteins may be involved in perivascular tissue localization of human mast cells in vivo. |
| Document Type: Article |
| Language: English |
| Reprint Address: COLUMBO, M (reprint author), UNIV NAPLES FEDERICO II, FAC MED & CHIRURG, SCH MED, DIV CLIN IMMUNOL, CATTEDRA IMMUNOL CLIN, I-80131 NAPLES, ITALY |
Addresses:
1. JOHNS HOPKINS UNIV, SCH MED, DEPT MED, DIV CLIN IMMUNOL, BALTIMORE, MD 21224 USA |
| Publisher: AMER ASSOC IMMUNOLOGISTS, 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 |
| Subject Category: Immunology |
| IDS Number: RB197 |
| ISSN: 0022-1767 |
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