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| STRUCTURE OF BACTERIAL FLAGELLAR FILAMENTS AT 11 ANGSTROM RESOLUTION - PACKING OF THE ALPHA-HELICES |
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| Author(s): MORGAN DG, OWEN C, MELANSON LA, DEROSIER DJ |
| Source: JOURNAL OF MOLECULAR BIOLOGY Volume: 249 Issue: 1 Pages: 88-110 Published: MAY 26 1995 |
| Times Cited: 100 References: 49 |
| Abstract: Recent advances in the analysis of electron micrographs of frozen, hydrated bacterial filaments have allowed us to average data from more than 150 images and to reconstruct the bacterial flagellar filament of Salmonella typhimurium at a resolution of similar to 11 Angstrom. In addition to the outermost features seen in earlier lower resolution maps of the filament, we find a pair of concentric tubes which surround a similar to 30 Angstrom diameter channel at the center of the structure. The walls of these tubes are composed of rod-like features which we have interpreted as columns of individual alpha-helices stacked end-to-end. Each column runs approximately parallel to the helix axis. The wall of the innermost tube, at a radius of similar to 20 Angstrom, is formed from II such columns. The wall of the second tube is formed from 22 columns which occur alternately at radii of similar to 43 and similar to 47 Angstrom. The two concentric tubes are held apart by spacers. These are short, rod-like features, which run approximately parallel to the helix axis. We have interpreted these as additional cl-helices. By symmetry each flagellin monomer contributes an alpha-helix to the inner tube, two alpha-helices to the outer tube and a fourth alpha-helix to the spacer. We have tentatively assigned one type of alpha-helix in the outer tube to the similar to 30 C-terminal residues of flagellin while the remaining three alpha-helices are assigned to the similar to 70 N-terminal residues. This interpretation of the reconstruction is consistent with available biochemical, biophysical and amino acid sequence information. We also present details of improved methodology to extract and evaluate the original data and also to assess the statistical significance of features in the three-dimensional map.
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| Document Type: Article |
| Language: English |
| Reprint Address: MORGAN, DG (reprint author), BRANDEIS UNIV, ROSENSTIEL BASIC MED SCI RES CTR, WALTHAM, MA 02254 USA |
| Publisher: ACADEMIC PRESS (LONDON) LTD, 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX |
| Subject Category: Biochemistry & Molecular Biology |
| IDS Number: RC665 |
| ISSN: 0022-2836 |
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