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| IDENTIFICATION AND INHIBITION OF THE ICE/CED-3 PROTEASE NECESSARY FOR MAMMALIAN APOPTOSIS |
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| Author(s): NICHOLSON DW, ALI A, THORNBERRY NA, VAILLANCOURT JP, DING CK, GALLANT M, GAREAU Y, GRIFFIN PR, LABELLE M, LAZEBNIK YA, MUNDAY NA, RAJU SM, SMULSON ME, YAMIN TT, YU VL, MILLER DK |
| Source: NATURE Volume: 376 Issue: 6535 Pages: 37-43 Published: JUL 6 1995 |
| Times Cited: 2,780 References: 48 |
| Abstract: The protease responsible for the cleavage of poly(ADP-ribose) polymerase and necessary for apoptosis has been purified and characterized. This enzyme, named apopain, is composed of two subunits of relative molecular mass (M(r)) 17K and 12K that are derived from a common proenzyme identified as CPP32. This proenzyme is related to interleukin-1 beta-converting enzyme (ICE) and CED-3, the product of a gene required for programmed cell death in Caenorhabditis elegans. A potent peptide aldehyde inhibitor has been developed and shown to prevent apoptotic events In vitro, suggesting that apopain/CPP32 is important for the initiation of apoptotic cell death. |
| Document Type: Article |
| Language: English |
| Reprint Address: NICHOLSON, DW (reprint author), MERCK FROSST CANADA INC, MERCK FROSST CTR THERAPEUT RES, DEPT BIOCHEM & MOLEC BIOL, POB 1005, POINTE CLAIRE, PQ H9R 4P8 CANADA |
Addresses:
1. MERCK FROSST CANADA INC, MERCK FROSST CTR THERAPEUT RES, DEPT MED CHEM, POINTE CLAIRE, PQ H9R 4P8 CANADA
2. MERCK & CO INC, RES LABS, DEPT BIOCHEM, RAHWAY, NJ 07065 USA
3. MERCK & CO INC, RES LABS, DEPT INFLAMMAT RES, RAHWAY, NJ 07065 USA
4. COLD SPRING HARBOR LAB, COLD SPRING HARBOR, NY 11724 USA
5. GEORGETOWN UNIV, SCH MED, DEPT BIOCHEM & MOLEC BIOL, WASHINGTON, DC USA |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: RH111 |
| ISSN: 0028-0836 |
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| | | | | | | | | Record from Web of Science® | | | | | | | | | |