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| AN ANTAGONIST OF INTEGRIN ALPHA(V)BETA(3) PREVENTS MATURATION OF BLOOD-VESSELS DURING EMBRYONIC NEOVASCULARIZATION |
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| Author(s): DRAKE CJ, CHERESH DA, LITTLE CD |
| Source: JOURNAL OF CELL SCIENCE Volume: 108 Pages: 2655-2661 Part: Part 7 Published: JUL 1995 |
| Times Cited: 204 References: 28 |
| Abstract: Experimental data in this study demonstrate that integrin alpha(v) beta(3) is fundamentally involved in the maturation of blood vessels during embryonic neovascularization (vasculogenesis), Integrin alpha(v) beta(3) was specifically expressed on the surface of angioblasts during vessel development in quail embryos and vitronectin, a ligand for alpha(v) beta(3), localized to the basal surface of these cells, More importantly, microinjection of the anti-alpha(v) beta(3) monoclonal antibody, LM609, disrupted the normal pattern of vascular development. After exposure to LM609 the angioblasts in experimental embryos appeared as clusters of rounded cells lacking normal cellular protrusions. This led to disruption of lumen formation and abnormal vessel patterning. These findings demonstrate that during vasculogenesis ligation of integrin alpha(v) beta(3) on the surface of primordial endothelial cells is critical for the differentiation and maturation of blood vessels, Similar studies on chicken chorioallantoic membrane showed that LM609 blocks angiogenesis. Together the two studies suggest that integrin alpha(v) beta(3) plays a role in neovascularization of tissues. |
| Document Type: Article |
| Language: English |
Addresses:
1. MED UNIV S CAROLINA, DEPT CELL BIOL, CHARLESTON, SC 29425 USA
2. MED UNIV S CAROLINA, CTR CARDIOVASC DEV BIOL, CHARLESTON, SC 29425 USA
3. Scripps Res Inst, DEPT IMMUNOL, LA JOLLA, CA 92037 USA
4. Scripps Res Inst, DEPT VASC BIOL, LA JOLLA, CA 92037 USA |
| Publisher: COMPANY OF BIOLOGISTS LTD, BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL |
| Subject Category: Cell Biology |
| IDS Number: RJ382 |
| ISSN: 0021-9533 |
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