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| ELEVATED BODY SWING TEST - A NEW BEHAVIORAL PARAMETER FOR RATS WITH 6-HYDROXYDOPAMINE-INDUCED HEMIPARKINSONISM |
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| Author(s): BORLONGAN CV, SANBERG PR |
| Source: JOURNAL OF NEUROSCIENCE Volume: 15 Issue: 7 Pages: 5372-5378 Part: Part 2 Published: JUL 1995 |
| Times Cited: 81 References: 47 |
| Abstract: Parkinson's disease is characterized by a depletion of dopamine (DA) neurons in the nigrostriatal pathway, Stereotaxic injections of 6-hydroxydopamine (6-OHDA), a selective neurotoxin, into either the medial forebrain bundle or the substantia nigra result in a massive DA denervation of the nigrostriatal pathway, Following unilateral nigrostriatal DA depletion, hemiparkinsonian animals develop a stereotypical rotational behavior when challenged with DA agonists such as apomorphine. The drug-induced rotational behavior has been widely used as the behavioral index of hemiparkinsonian animals, but it has some limitations, Although asymmetries in the rotational behavior may indicate an imbalance of DA contents and release capacity in the bilateral nigrostriatal pathway, the behavior is a pharmacological reaction, Accordingly, the drug-induced rotation test is subject to sensitization effects, The present study proposes the elevated body swing test (EBST) as a measure of asymmetrical motor behavior of hemiparkinsonian animals in a drug-free state, The EBST simply involves elevating the animal by handling its tail and recording the frequency and direction of the swing behavior, Unilateral nigral 6-OHDA-lesioned rats exhibited significant biased swing activity with the direction contralateral to the lesioned side, corresponding to the direction of apomorphine-induced rotations, A 30 sec EBST was noted as the peak time for biased swing activity, At 7 d postlesion (the start of testing), and every week thereafter for a period of 2 months, a fairly stable biased swing activity level was observed, At 1 and 2 months postlesion, the same animals were also challenged with apomorphine. High positive correlations between swing and apomorphine-induced rotational behavior were noted, Furthermore, tail pinch or apomorphine injection increased the level of biased swing activity in the lesioned animals, Similar mechanisms implicated in the dopamine-mediated rotational behavior may be involved in the swing behavior, The EBST may circumvent the problem of sensitization and pose as an alternative tool in characterizing spontaneous behavior in animals with lesions of the nigrostriatal pathway. |
| Document Type: Article |
| Language: English |
| Reprint Address: BORLONGAN, CV (reprint author), UNIV S FLORIDA, COLL MED, DEPT SURG, DIV NEUROL SURG, 12901 BRUCE B DOWNS BLVD, MDC BOX 16, TAMPA, FL 33612 USA |
Addresses:
1. UNIV S FLORIDA, COLL MED, DEPT NEUROL, DIV NEUROL SURG, TAMPA, FL 33612 USA
2. UNIV S FLORIDA, COLL MED, DEPT PSYCHIAT, DIV NEUROL SURG, TAMPA, FL 33612 USA
3. UNIV S FLORIDA, COLL MED, DEPT PHARMACOL, DIV NEUROL SURG, TAMPA, FL 33612 USA |
| Publisher: OXFORD UNIV PRESS INC, JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 |
| Subject Category: Neurosciences |
| IDS Number: RJ666 |
| ISSN: 0270-6474 |
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