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| SUBCELLULAR-LOCALIZATION OF AND PHOTOSENSITIZATION BY PROTOPORPHYRIN-IX IN HUMAN KERATINOCYTES AND FIBROBLASTS CULTIVATED WITH 5-AMINOLEVULINIC ACID |
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| Author(s): GAULLIER JM, GEZE M, SANTUS R, MELO TSE, MAZIERE JC, BAZIN M, MORLIERE P, DUBERTRET L |
| Source: PHOTOCHEMISTRY AND PHOTOBIOLOGY Volume: 62 Issue: 1 Pages: 114-122 Published: JUL 1995 |
| Times Cited: 46 References: 23 |
| Abstract: The subcellular localization of protoporphyrin (PP) has been studied by microspectrofluorometric techniques in NCTC 2544 keratinocytes incubated with 5-aminolevulinic acid (ALA) for times up to 42 h. Whereas the plasma membrane shows strong staining, fluorescent spots are observed within the cytoplasm especially in the perinuclear region. Although the topographic pattern of the PP distribution does not change much with the incubation time with ALA, the fluorescence spectra suggest that the PP microenvironments are quite different at short and long incubation times. Addition of 18 mu M desferrioxamine almost doubles the ALA-induced PP concentration. Colocalization experiments with rhodamine 123, a mitochondrial probe, and lucifer yellow (LY) or neutral red (NR), two lysosome probes, demonstrate that at least some of these spots are of lysosomal origin. Study of the time evolution of the NR fluorescence under irradiation with visible light in the presence and absence of ALA demonstrates that lysosomes are damaged in cells that have synthesized PP. No PP fluorescence can be detected in mitochondria after incubation with ALA. However, photosensitization of mitochondria occurs under irradiation with visible light. Very little formation of lipofuscins by photosensitization with exogenous PP or ALA-induced PP is observed with the NCTC 2544 keratinocytes, as compared to normal human fibroblasts. |
| Document Type: Article |
| Language: English |
Addresses:
1. MUSEUM NATL HIST NAT, PHYSICOCHIM ADAPT BIOL LAB, INSERM, U312, F-75231 PARIS 05, FRANCE
2. UTL, INST SUPER TECN, P-1096 LISBON, PORTUGAL
3. HOP ST LOUIS, DERMATOL LAB, INSERM, U312, F-75010 PARIS, FRANCE |
| Publisher: AMER SOC PHOTOBIOLOGY, BIOTECH PARK, 1021 15TH ST, SUITE 9, AUGUSTA, GA 30901-3158 |
| Subject Category: Biochemistry & Molecular Biology; Biophysics |
| IDS Number: RJ710 |
| ISSN: 0031-8655 |
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