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| ALLELOTYPING OF DUCTAL CARCINOMA IN-SITU OF THE BREAST - DELETION OF LOCI ON 8P, 13Q, 16Q, 17P AND 17Q |
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| Author(s): RADFORD DM, FAIR KL, PHILLIPS NJ, RITTER JH, STEINBRUECK T, HOLT MS, DONISKELLER H |
| Source: CANCER RESEARCH Volume: 55 Issue: 15 Pages: 3399-3405 Published: AUG 1 1995 |
| Times Cited: 156 References: 58 |
| Abstract: In order to determine which tumor suppressor loci are involved in preinvasive breast cancer, we have assayed for loss of heterozygosity (LOH) in ductal carcinoma in situ (DCIS). Areas of DCIS were microdissected from archival paraffin-embedded tissue. DNA was extracted, and LOH was determined by PCR of microsatellite markers that map to 39 autosomal arms. Either uninvolved lymph node or white cell DNA was used as normal control. A total of 61 samples of DCIS were assayed. The average number of informative tumors examined for each marker was 19 (range, 8-48). The median fractional allelic loss was 0.037. The highest percentage of LOH was shown for loci on 8p (18.7%), 13q (18%), 16q (28.6%), 17p (37.5%), and 17q (15.9%). LOH on 18q was found in 10.7% of informative tumors. Fractional allelic loss was associated with LOH on 17p, with high nuclear grade and with the comedo subtype of DCIS. LOH on 17p correlated with LOH on 17q and on 13q. Additional markers were used for 16q and 17p to determine the smallest common region of deletion. These data provide evidence that tumor suppressor loci that map to these regions are involved in the oncogenesis of breast cancer before progression to the invasive phenotype. Our findings provide additional support that multiple loci on 17p and 16q are involved in the development of breast cancer. |
| Document Type: Article |
| Language: English |
| Reprint Address: RADFORD, DM (reprint author), WASHINGTON UNIV, DEPT SURG, DIV HUMAN MOLEC GENET, 660 S EUCLID AVE, 9901 WOHL HOSP, ST LOUIS, MO 63110 USA |
Addresses:
1. WASHINGTON UNIV, DEPT PATHOL, ST LOUIS, MO 63110 USA
2. ST LOUIS UNIV, DEPT PATHOL, ST LOUIS, MO 63110 USA |
| Publisher: AMER ASSOC CANCER RESEARCH, PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 |
| Subject Category: Oncology |
| IDS Number: RL493 |
| ISSN: 0008-5472 |
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