| | |  | | | | Record from Web of Science® | | | | | | |
| A NEW FAMILY OF OUTWARDLY RECTIFYING POTASSIUM CHANNEL PROTEINS WITH 2 PORE DOMAINS IN TANDEM |
|
|
| Author(s): KETCHUM KA, JOINER WJ, SELLERS AJ, KACZMAREK LK, GOLDSTEIN SAN |
| Source: NATURE Volume: 376 Issue: 6542 Pages: 690-695 Published: AUG 24 1995 |
| Times Cited: 238 References: 24 |
| Abstract: POTASSIUM channels catalyse the permeation of K+ ions across cellular membranes and are identified by a common structural moth, a highly conserved signature sequence of eight amino acids in the P domain of each channel's pore-forming alpha-subunit(1,2), Here we describe a novel K+ channel (TOK1) from Saccharomyces cerevisiae that contains two P domains within one continuous polypeptide, Xenopus laevis oocytes expressing the channel exhibit a unique, outwardly rectifying, K+-selective current. The channel is permeable to outward how of ions at membrane potentials above the K+ equilibrium potential; its conduction-voltage relationship is thus sensitive to extracellular K+ ion concentration. In excised membrane patches, external divalent cations block the channel in a voltage-dependent manner, and their removal in this configuration allows inward channel current, These attributes are similar to those described for inwardly rectifying K+ channels(3,4), but in the opposite direction, a previously uorecognized channel behaviour. Our results identify a new class of K+ channel which is distinctive in both its primary structure and functional properties. Structural homologues of the channel are present in the genome of Caenorhabditis elegans. |
| Document Type: Article |
| Language: English |
| Reprint Address: KETCHUM, KA (reprint author), YALE UNIV, SCH MED, BOYER CTR MOLEC MED, DEPT PEDIAT, 295 CONGRESS AVE, NEW HAVEN, CT 06536 USA |
Addresses:
1. YALE UNIV, SCH MED, BOYER CTR MOLEC MED, DEPT GENET, NEW HAVEN, CT 06536 USA
2. YALE UNIV, SCH MED, BOYER CTR MOLEC MED, DEPT PHARMACOL, NEW HAVEN, CT 06536 USA
3. YALE UNIV, SCH MED, BOYER CTR MOLEC MED, DEPT CELLULAR & MOLEC PHYSIOL, NEW HAVEN, CT 06536 USA |
| Publisher: MACMILLAN MAGAZINES LTD, 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF |
| Subject Category: Multidisciplinary Sciences |
| IDS Number: RQ672 |
| ISSN: 0028-0836 |
|
| | | | | | | | | Record from Web of Science® | | | | | | | | | |