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| TUMORIGENICITY OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE I-INFECTED CELL-LINES IN SEVERE COMBINED IMMUNODEFICIENT MICE AND CHARACTERIZATION OF THE CELLS PROLIFERATING IN-VIVO |
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| Author(s): IMADA K, TAKAORIKONDO A, AKAGI T, SHIMOTOHNO K, SUGAMURA K, HATTORI T, YAMABE H, OKUMA M, UCHIYAMA T |
| Source: BLOOD Volume: 86 Issue: 6 Pages: 2350-2357 Published: SEP 15 1995 |
| Times Cited: 43 References: 49 |
| Abstract: The mechanism involved in leukemogenesis and neoplastic cell growth of adult T-cell leukemia (ATL) still remains unclear, We examined the tumorigenicity of human T-cell leukemia virus type I (HTLV-I)-infected cell lines in an in vivo cell proliferation model using severe combined immunodeficient (SCID) mice. Eleven HTLV-I-infected cell lines were injected into SCID mice and we found that 4 of them were capable of proliferating in SCID mice. Three of four transplantable cell lines are derived from the leukemic cell clone and 6 of 6 HTLV-I-infected cell lines of nonleukemic cell origin could not engraft in SCID mice. Interestingly, it was shown that some HTLV-I-infected and interleukin-2 (IL-2)-dependent cell lines could successfully engraft in SCID mice. The expression of IL-2 mRNA was not detected in these cell lines growing either in vivo or in vitro. HTLV-I viral products were not detected in 3 of 4 transplantable cell lines proliferating in vivo. Peripheral blood T cells immortalized by introduction of tax gene of HTLV I were found to have no tumorigenic potential in SCID mice. These data suggest that (1) HTLV-I-infected cell lines of nonleukemic cell origin do not have enough leukemogenic changes to acquire the tumorigenic potential in SCID mice; (2) the IL-2 autocrine mechanism is not directly involved in the tumor cell growth; (3) viral gene expression is not needed for the maintenance of neoplastic cell growth; and (4) the expression of tax gene is not sufficient for the neoplastic cell growth in vivo. (C) 1995 by The American Society of Hematology. |
| Document Type: Article |
| Language: English |
Addresses:
1. KYOTO UNIV, INST VIRUS RES, DEPT INTERNAL MED 1, SAKYO KU, KYOTO 606, JAPAN
2. KYOTO UNIV, ANAT PATHOL LAB, KYOTO 606, JAPAN
3. NATL CANC CTR, RES INST, DIV VIROL, TOKYO 104, JAPAN
4. TOHOKU UNIV, SCH MED, DEPT MICROBIOL, SENDAI, MIYAGI 980 JAPAN |
| Publisher: W B SAUNDERS CO, INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 |
| Subject Category: Hematology |
| IDS Number: RU052 |
| ISSN: 0006-4971 |
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