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| EFFECTS OF MONOCLONAL ANTI-C-KIT ANTIBODY (ACK2) ON MELANOCYTES IN NEWBORN MICE |
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| Author(s): OKURA M, MAEDA H, NISHIKAWA S, MIZOGUCHI M |
| Source: JOURNAL OF INVESTIGATIVE DERMATOLOGY Volume: 105 Issue: 3 Pages: 322-328 Published: SEP 1995 |
| Times Cited: 44 References: 18 |
| Abstract: Previous studies indicate that c-Kit is required for postnatal melanocyte development, To understand the precise mechanisms of c-Kit dependence, we studied melanocyte development in newborn C57BL/6 mice by means of peritoneal injection of a monoclonal anti-c-Kit antibody (ACK2), which blocks c-Kit functions. The mice were injected once or more with ACK2 at various intervals after birth. In experiment 1, skin samples were examined on day 10 post-partum and in experiment 2 they were examined daily until day 10 post-partum, We studied melanocytes in the hair follicles, epidermis, and dermis by light and electron microscopy with dopa reactions and immunohistochemistry. Epidermal melanocytes in untreated mice were dopa negative and c-Kit positive on day 0 post-partum but became dopa positive soon thereafter, In ACK2-treated mice, the earlier the mice received ACK2 injections after birth, the fewer melanocytes they had, not only in the epidermis, but also in follicles. In these mice, melanocytes that had undergone apoptosis in the dermis and the follicles were detected ultrastructurally. Some appeared to have produced tyrosinase, because they had dopa-positive melanosomes, These results suggest that melanocytes in newborn mice are c-Kit dependent and undergo apoptosis when c-Kit receptors are blocked by ACK2 in the early days after birth. During this c-Kit-dependent period, melanocytes differentiate from dopa negative to positive and migrate from the epidermis to hair follicles. |
| Document Type: Article |
| Language: English |
| Reprint Address: OKURA, M (reprint author), ST MARIANNA UNIV, SCH MED, DEPT DERMATOL, MIYAMAE KU, 2-16-1 SUGAO, KAWASAKI, KANAGAWA JAPAN |
Addresses:
1. KUMAMOTO UNIV, SCH MED, INST MED IMMUNOL, DEPT PATHOL, KUMAMOTO 860, JAPAN
2. KYOTO UNIV, FAC MED, DEPT MOLEC GENET, KYOTO, JAPAN |
| Publisher: BLACKWELL SCIENCE PUBL INC CAMBRIDGE, 238 MAIN ST, CAMBRIDGE, MA 02142 |
| Subject Category: Dermatology |
| IDS Number: RU399 |
| ISSN: 0022-202X |
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