| THE T-PROTEIN ENCODED BY BRACHYURY IS A TISSUE-SPECIFIC TRANSCRIPTION FACTOR |
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| Author(s): KISPERT A, KOSCHORZ B, HERRMANN BG |
| Source: EMBO JOURNAL Volume: 14 Issue: 19 Pages: 4763-4772 Published: OCT 2 1995 |
| Times Cited: 167 References: 51 |
| Abstract: The mouse Brachyury (T) gene is required for differentiation of the notochord and formation of mesoderm during posterior development. Homozygous embryos lacking T activity do not develop a trunk and tail and die in utero. The T gene is specifically expressed in notochord and early mesoderm cells in the embryo. Recent data have demonstrated that the T protein is localized in the cell nucleus and specifically binds to a palindrome of 20 bp (the T site) in vitro. We show that the T protein activates expression of a reporter gene in HeLa cells through binding to the T site, Thus T is a novel tissue-specific transcription factor, It consists of a large N-terminal DNA binding domain (amino acids 1-229) and two pairs of transactivation and repression domains in the C-terminal protein half, T can also transactivate transcription through variously oriented and spaced T sites, a fact that may be relevant in the search for genes controlled by T protein and important in mesoderm development. |
| Document Type: Article |
| Language: English |
Addresses:
1. MAX PLANCK INST ENTWICKLUNGSBIOL, BIOCHEM ABT, D-72076 TUBINGEN, GERMANY |
| Publisher: OXFORD UNIV PRESS UNITED KINGDOM, WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP |
| Subject Category: Biochemistry & Molecular Biology; Cell Biology |
| IDS Number: TA216 |
| ISSN: 0261-4189 |